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Protein

Bifunctional enzyme LpxC/FabZ

Gene

lpxC/fabZ

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.
Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.By similarity

Catalytic activityi

UDP-3-O-((3R)-3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine + H2O = UDP-3-O-((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + acetate.
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.

Cofactori

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. Bifunctional enzyme LpxC/FabZ (lpxC/fabZ)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi79 – 791Zinc; via tele nitrogenBy similarity
Metal bindingi264 – 2641Zinc; via tele nitrogenBy similarity
Metal bindingi268 – 2681ZincBy similarity
Active sitei291 – 2911Proton donorBy similarity
Active sitei370 – 3701By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-1701-MONOMER.
UniPathwayiUPA00359; UER00478.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme LpxC/FabZ
Including the following 2 domains:
UDP-3-O-acyl-N-acetylglucosamine deacetylase (EC:3.5.1.108)
Short name:
UDP-3-O-acyl-GlcNAc deacetylase
Alternative name(s):
UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ (EC:4.2.1.59)
Alternative name(s):
(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase
Short name:
(3R)-hydroxymyristoyl-ACP dehydrase
Beta-hydroxyacyl-ACP dehydratase
Gene namesi
Name:lpxC/fabZ
Ordered Locus Names:CT1662
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Bifunctional enzyme LpxC/FabZPRO_0000191974Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi194439.CT1662.

Structurei

3D structure databases

ProteinModelPortaliQ8KBX0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 306306UDP-3-O-acyl-N-acetylglucosamine deacetylaseAdd
BLAST
Regioni307 – 4671613-hydroxyacyl-[acyl-carrier-protein] dehydrataseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the LpxC family.Curated
In the C-terminal section; belongs to the thioester dehydratase family.Curated

Phylogenomic databases

eggNOGiENOG4105C7C. Bacteria.
COG0764. LUCA.
COG0774. LUCA.
HOGENOMiHOG000029820.
KOiK16363.
OMAiRTFSFLH.
OrthoDBiPOG091H0292.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
3.30.1700.10. 1 hit.
3.30.230.20. 1 hit.
HAMAPiMF_00406. FabZ. 1 hit.
MF_00388. LpxC. 1 hit.
InterProiIPR013114. FabA_FabZ.
IPR010084. FabZ.
IPR029069. HotDog_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR004463. UDP-acyl_GlcNac_deAcase.
IPR011334. UDP-acyl_GlcNac_deAcase_C.
IPR015870. UDP-acyl_N-AcGlcN_deAcase_N.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
PF03331. LpxC. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 3 hits.
SSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01750. fabZ. 1 hit.
TIGR00325. lpxC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KBX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIHQRTLQN EISLTGIGLH TGHECTITFK PAPVNTGYIF VRTDINDCPE
60 70 80 90 100
IPALIDHVVD VLRGTTIGIG DVKVHTTEHV LAALYGLQID NCRIELSGPE
110 120 130 140 150
PPVLDGSSNP FAEALLSAGI AEQDEPKNYL VIDETIEFHN PEKSVDIVAL
160 170 180 190 200
PLDGFRMTVM VDYKNPALGS QHSGLFDLDK EFLREFSPCR TFCFLSEVEA
210 220 230 240 250
MANQGIIKGA DIDNAIVIVD KQLDETEVQT LADKVGVDAS HLVLGQNGIL
260 270 280 290 300
NNRELRFSNE PARHKLLDLL GDLALLGMPV KAQILAXRPG HASNVEFVKQ
310 320 330 340 350
LKKYADRNKL ARQYQHEKKA GVIFDINAIQ NILPHRYPFL LIDKIVEFKL
360 370 380 390 400
DEKIVSIKNV TMNEPFFQGH FPGNPIMPGV LIIEAMAQTG GIMMLNGKEN
410 420 430 440 450
IKESVVFFMG IDKARFRKPV LPGDTLVIEA VMTNMRRTVC QFDAKAYVRG
460
ELVCEASLMA TVMEKKN
Length:467
Mass (Da):51,844
Last modified:October 1, 2002 - v1
Checksum:i355120C7D2B654E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM72887.1.
RefSeqiNP_662545.1. NC_002932.3.
WP_010933326.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM72887; AAM72887; CT1662.
GeneIDi1006145.
KEGGicte:CT1662.
PATRICi21401191. VBIChlTep116050_1500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM72887.1.
RefSeqiNP_662545.1. NC_002932.3.
WP_010933326.1. NC_002932.3.

3D structure databases

ProteinModelPortaliQ8KBX0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT1662.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM72887; AAM72887; CT1662.
GeneIDi1006145.
KEGGicte:CT1662.
PATRICi21401191. VBIChlTep116050_1500.

Phylogenomic databases

eggNOGiENOG4105C7C. Bacteria.
COG0764. LUCA.
COG0774. LUCA.
HOGENOMiHOG000029820.
KOiK16363.
OMAiRTFSFLH.
OrthoDBiPOG091H0292.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00478.
BioCyciCTEP194439:GHN0-1701-MONOMER.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
3.30.1700.10. 1 hit.
3.30.230.20. 1 hit.
HAMAPiMF_00406. FabZ. 1 hit.
MF_00388. LpxC. 1 hit.
InterProiIPR013114. FabA_FabZ.
IPR010084. FabZ.
IPR029069. HotDog_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR004463. UDP-acyl_GlcNac_deAcase.
IPR011334. UDP-acyl_GlcNac_deAcase_C.
IPR015870. UDP-acyl_N-AcGlcN_deAcase_N.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
PF03331. LpxC. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 3 hits.
SSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01750. fabZ. 1 hit.
TIGR00325. lpxC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXZ_CHLTE
AccessioniPrimary (citable) accession number: Q8KBX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.