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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Metal bindingi67MagnesiumUniRule annotation1
Metal bindingi140MagnesiumUniRule annotation1
Metal bindingi143MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-1703-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:CT1664
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001830891 – 186Crossover junction endodeoxyribonuclease RuvCAdd BLAST186

Interactioni

Protein-protein interaction databases

STRINGi194439.CT1664

Structurei

3D structure databases

ProteinModelPortaliQ8KBW8
SMRiQ8KBW8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012181
KOiK01159
OMAiGLTRCGI
OrthoDBiPOG091H0202

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KBW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVLGIDPGS RKTGYGVIAE TAAGYRVLGC GLVRPRAADT LHERISQLCA
60 70 80 90 100
GLDEVIEQLK PEAVALETAF VGRNVRSALI LGQVRGAVLA TVMRHSLPVR
110 120 130 140 150
EYAPREIKLS VTGTGSACKE QVAAMLSRML ELGGELKPLD VTDALGIAYC
160 170 180
DLARGASSLG GQLRKNGKGR SKGWAAFVNE HPELMA
Length:186
Mass (Da):19,695
Last modified:October 1, 2002 - v1
Checksum:i2B8082DBC0BAF9FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM72889.1
RefSeqiNP_662547.1, NC_002932.3
WP_010933328.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM72889; AAM72889; CT1664
GeneIDi1007091
KEGGicte:CT1664
PATRICifig|194439.7.peg.1502

Similar proteinsi

Entry informationi

Entry nameiRUVC_CHLTE
AccessioniPrimary (citable) accession number: Q8KBW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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