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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi287Magnesium or manganeseUniRule annotation1
Metal bindingi289Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 198ATPUniRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-1713-MONOMER
UniPathwayiUPA00074; UER00125

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:CT1674
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514421 – 425Phosphoribosylamine--glycine ligaseAdd BLAST425

Proteomic databases

PRIDEiQ8KBV8

Interactioni

Protein-protein interaction databases

STRINGi194439.CT1674

Structurei

3D structure databases

ProteinModelPortaliQ8KBV8
SMRiQ8KBV8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini110 – 317ATP-graspUniRule annotationAdd BLAST208

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QNG Bacteria
COG0151 LUCA
HOGENOMiHOG000033463
KOiK01945
OMAiKATVCKY
OrthoDBiPOG091H02DZ

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.90.600.10, 1 hit
HAMAPiMF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KBV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLIIGSGA REHAMAWAVA RSSKVSTVFV APGNGGTATM GGKVRNTPVK
60 70 80 90 100
ATDIDALLEL VAKESIGLTV VGPEQPLEAG IVNRFREAGF KVVGPTAEAA
110 120 130 140 150
QLETSKVFAK EFMKRHGIPT AGYEVFRDYA SAKAFLETCP TFPQVIKASG
160 170 180 190 200
LCAGKGVVVA MSRDEALEAI HEFFESRIFG DAADEVVIEA FLSGQEASVF
210 220 230 240 250
ALTDGQNYQL FLSAQDHKRI GEGDTGKNTG GMGAYAPAPL VTPEVMRRVE
260 270 280 290 300
EEVIRPTLAG MRADGYAYTG FLYVGLMIDK GVPSVVEYNA RLGDPETQVV
310 320 330 340 350
LPMLKSDLFD ALLASVEGGL EVVPFEMQEG AAATVVMASA GYPDAYETGK
360 370 380 390 400
VITIDPTVND MEGVLVFHAG TRRDGDALVT SGGRVLSVTA CAGSLKEALD
410 420
RVYRAVDAIE FEGAYCRRDI GAKAL
Length:425
Mass (Da):45,064
Last modified:October 1, 2002 - v1
Checksum:i8C6228E97721FA1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM72899.1
RefSeqiNP_662557.1, NC_002932.3
WP_010933338.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM72899; AAM72899; CT1674
GeneIDi1006158
KEGGicte:CT1674
PATRICifig|194439.7.peg.1512

Similar proteinsi

Entry informationi

Entry nameiPUR2_CHLTE
AccessioniPrimary (citable) accession number: Q8KBV8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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