Reviewed, UniProtKB/Swiss-Prot Q8KBV8 (PUR2_CHLTE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	CT1674

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151442
Regions
	Domain	110 – 317	208	ATP-grasp
	Nucleotide binding	137 – 198	62	ATP By similarity
Sites
	Metal binding	287	1	Magnesium or manganese By similarity
	Metal binding	289	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KBV8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 8C6228E97721FA1C	FASTA	425	45,064
	10 20 30 40 50 60 MKVLIIGSGA REHAMAWAVA RSSKVSTVFV APGNGGTATM GGKVRNTPVK ATDIDALLEL 70 80 90 100 110 120 VAKESIGLTV VGPEQPLEAG IVNRFREAGF KVVGPTAEAA QLETSKVFAK EFMKRHGIPT 130 140 150 160 170 180 AGYEVFRDYA SAKAFLETCP TFPQVIKASG LCAGKGVVVA MSRDEALEAI HEFFESRIFG 190 200 210 220 230 240 DAADEVVIEA FLSGQEASVF ALTDGQNYQL FLSAQDHKRI GEGDTGKNTG GMGAYAPAPL 250 260 270 280 290 300 VTPEVMRRVE EEVIRPTLAG MRADGYAYTG FLYVGLMIDK GVPSVVEYNA RLGDPETQVV 310 320 330 340 350 360 LPMLKSDLFD ALLASVEGGL EVVPFEMQEG AAATVVMASA GYPDAYETGK VITIDPTVND 370 380 390 400 410 420 MEGVLVFHAG TRRDGDALVT SGGRVLSVTA CAGSLKEALD RVYRAVDAIE FEGAYCRRDI GAKAL

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM72899.1.
RefSeq	NP_662557.1.
3D structure databases
SMR	Q8KBV8. Positions 1-425.
ModBase	Search...
Genome annotation databases
GeneID	1006158.
GenomeReviews	Gene locus CT1674 in contig AE006470_GR.
KEGG	cte:CT1674.
NMPDR	fig|194439.1.peg.1651.
TIGR	CT1674.
Phylogenomic databases
HOGENOM	HBG404060.
OMA	FSLMAFV.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1674-MONOMER.
BRENDA	6.3.4.13. 189605.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_CHLTE

Accession

Primary (citable) accession number: Q8KBV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)