Q8KBF7 (KDSB_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase
EC=2.7.7.38

Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase
Short name=CKS
Short name=CMP-KDO synthase

Gene names

Name:	kdsB
Ordered Locus Names:	CT1830

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity. HAMAP MF_00057
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00057
Subcellular location	Cytoplasm Potential HAMAP MF_00057.
Sequence similarities	Belongs to the KdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057		PRO_0000370046

Sequences

	Sequence		Length	Mass (Da)
	Q8KBF7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 74F0437F1019CEBC	FASTA	248	27,730
	10 20 30 40 50 60 MNAVIVIPAR LSSSRLKEKM LADLEGEPLI VRTWQQATKS RLASRVVVAT DSERIFAVLR 70 80 90 100 110 120 EAGAEVVMTS PDLTCGTDRI AEAAEQVGGD VFVNLQGDEP LIDPATIDLA IAPFFGEGPL 130 140 150 160 170 180 PDCTTLVFPL KPDERQIIDD PHVVKAVLDT RGNALYFSRS PIPYRRETLP DTKYYRHIGL 190 200 210 220 230 240 YAFRADVLKA FVALPPSMLE RAESLEQLRL LENGYRIRCI ETTTDTPGVN TEEELEEVRR LFRERFGA

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM73051.1.
RefSeq	NP_662709.1. NC_002932.3.
3D structure databases
HSSP	HSSP built from PDB template 1VH3 based on UniProtKB P44490.
ProteinModelPortal	Q8KBF7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1008090.
GenomeReviews	Gene locus CT1830 in contig AE006470_GR.
KEGG	cte:CT1830.
NMPDR	fig|194439.1.peg.1803.
PATRIC	21401515. VBIChlTep116050_1662.
TIGR	CT1830.
Phylogenomic databases
HOGENOM	HBG637773.
OMA	INVQADE.
ProtClustDB	PRK05450.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1830-MONOMER.
Family and domain databases
HAMAP	MF_00057. CMP-KDO_synth. [Tree]
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
KO	K00979.
PANTHER	PTHR21485:SF4. PTHR21485:SF4. 1 hit.
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. KdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_CHLTE

Accession

Primary (citable) accession number: Q8KBF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program