Q8KB71 (AROA_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	CT1918

Organism

Chlorobium tepidum

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088245

Sequences

	Sequence		Length	Mass (Da)
	Q8KB71 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6AFFA19D8C243C0E	FASTA	434	46,626
	10 20 30 40 50 60 MSTFQGEVTT LPPDKSISHR AALIGALAEG TTEISNFSGG YDNQSTLSVL RDAGISIRQE 70 80 90 100 110 120 ELSAGDGRIE RRVVIESNGL WSFREPSVPL MCNNSGSTMR MMAGIMAAQP FRSELVGDAS 130 140 150 160 170 180 LMKRPMKRVA DPLRQMGAEI SLSDAGTAPV VIHGTKALKT IEYRLPVPSA QVKSLVAFAA 190 200 210 220 230 240 LHADGQSKII EPIRSRDHTE LMLGLATIDR PDGVREIIID GRKPIAAKPF KVPADPSAAC 250 260 270 280 290 300 FMIALGLLGE RSEIVLRNVC LNPTRVAYID VLQEAGAGLG IENVRSEGGE PVGDIVVRSC 310 320 330 340 350 360 SGLKPLRISD HGVVAGVIDE VPMLAVLSAF ASGEFELHNA AELRTKESDR IDALVVNLQR 370 380 390 400 410 420 LGFECEQYAD GFVVKGRKTV ASEEVEIECF DDHRIAMSFT IAAEAAGASL RLSDRDVAGV 430 SFPNFFALID SLRQ

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM73137.1.
RefSeq	NP_662795.1. NC_002932.3.
3D structure databases
ProteinModelPortal	Q8KB71.
ModBase	Search...
Proteomic databases
PRIDE	Q8KB71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1007846.
GenomeReviews	Gene locus CT1918 in contig AE006470_GR.
KEGG	cte:CT1918.
NMPDR	fig|194439.1.peg.1889.
PATRIC	21401667. VBIChlTep116050_1737.
TIGR	CT1918.
Phylogenomic databases
HOGENOM	HBG646626.
OMA	LECANIS.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_1918-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. False negative. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_CHLTE

Accession

Primary (citable) accession number: Q8KB71

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program