Reviewed, UniProtKB/Swiss-Prot Q8KAZ0 (LPXA_CHLTE)

Names and origin

Protein names

Recommended name:
    Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
      Short name=UDP-N-acetylglucosamine acyltransferase
    EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	CT2008

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetylglucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine. HAMAP MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP MF_00387
Subunit structure	Homotrimer By similarity. HAMAP MF_00387
Subcellular location	Cytoplasm By similarity HAMAP MF_00387.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP MF_00387		PRO_0000188043

Sequences

	Sequence		Length	Mass (Da)
	Q8KAZ0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: FA17798BEB992EDF	FASTA	264	28,184
	10 20 30 40 50 60 MRNIHATAVI GSGAVLGEGV EIGPYTVIED DVVIGDRTVI GPHVHIADGA RIGNECRIST 70 80 90 100 110 120 GAVLATAPQD LKYAGEKTYL HIGDRTVIRE CVTLNRGTKA SGKTVVGSDN LIMAYVHAGH 130 140 150 160 170 180 DCVIGNHVVI ANSVQFGGHC HVGDYVVVGG LAGVHQXVRI GRYAMVGGIS RAALDVPPFV 190 200 210 220 230 240 MAGGHASFRY EGLNVIGLKR RGFTSEQLGN IRDAYRIIFQ SGLLLSKALE AVRNDLPQTP 250 260 EVVEILDFFA SGVYNRKFLK PFNS

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006470 Genomic DNA. Translation: AAM73226.1.
RefSeq	NP_662884.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1007553.
GenomeReviews	Gene locus CT2008 in contig AE006470_GR.
KEGG	cte:CT2008.
NMPDR	fig|194439.1.peg.1978.
TIGR	CT2008.
Phylogenomic databases
HOGENOM	HBG659295.
OMA	DLKYQGE.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_2008-MONOMER.
BRENDA	2.3.1.129. 189605.
Family and domain databases
HAMAP	MF_00387. LpxA. [Tree]
InterPro	IPR010137. Lipid_A_lpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
TIGRFAMs	TIGR01852. lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_CHLTE

Accession

Primary (citable) accession number: Q8KAZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)