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Q8KAX9 (DAPF_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diaminopimelate epimerase

Short name=DAP epimerase
EC=5.1.1.7
Gene names
Name:dapF
Ordered Locus Names:CT2021
OrganismChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS) [Reference proteome] [HAMAP]
Taxonomic identifier194439 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Diaminopimelate epimerase HAMAP-Rule MF_00197
PRO_0000149832

Regions

Region66 – 683Substrate binding By similarity
Region188 – 1892Substrate binding By similarity
Region199 – 2002Substrate binding By similarity

Sites

Active site661Proton donor/acceptor By similarity
Active site1981Proton donor/acceptor By similarity
Binding site61Substrate By similarity
Binding site391Substrate By similarity
Binding site571Substrate By similarity
Binding site1701Substrate By similarity
Site1381Important for catalytic activity By similarity
Site1881Important for catalytic activity By similarity

Amino acid modifications

Disulfide bond66 ↔ 198 HAMAP-Rule MF_00197

Sequences

Sequence LengthMass (Da)Tools
Q8KAX9 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: A25BD74CE3A5E9A5

FASTA25728,053
        10         20         30         40         50         60 
MSGAGNDFIV IDNRQGLFNL THEQVRAMCT RRTGIGADGL ILLETSETAD FRMNYHNADG 

        70         80         90        100        110        120 
FPGTMCGNGG RCAVWFAHLI GIRPTGKHYR FEAGPSTYEA EVTGEESVRL HMLPPSDFRD 

       130        140        150        160        170        180 
GLQAGAWNCH FVDTGSPHAI AYVNNLDQLD VLTEGGNIRH NKELFPDGTN VNFLEITAPD 

       190        200        210        220        230        240 
ALSIRTFERG VEDETLACGT GTVAAALMSF RLGKVTSSLV RVKVKSGETL MVGFNEMMDE 

       250 
IYLEGPARAV YRGTITL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006470 Genomic DNA. Translation: AAM73238.1.
RefSeqNP_662896.1. NC_002932.3.

3D structure databases

ProteinModelPortalQ8KAX9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING194439.CT2021.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM73238; AAM73238; CT2021.
GeneID1007540.
KEGGcte:CT2021.
PATRIC21401855. VBIChlTep116050_1831.

Phylogenomic databases

eggNOGCOG0253.
HOGENOMHOG000220466.
KOK01778.
OMALIVEPPY.
OrthoDBEOG6ND0M5.

Enzyme and pathway databases

BioCycCTEP194439:GHN0-2061-MONOMER.
UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERPTHR31689. PTHR31689. 1 hit.
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPF_CHLTE
AccessionPrimary (citable) accession number: Q8KAX9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways