Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateUniRule annotation
Binding sitei220 – 2201Substrate; via amide nitrogenUniRule annotation
Metal bindingi227 – 2271ManganeseUniRule annotation
Binding sitei227 – 2271SubstrateUniRule annotation
Metal bindingi247 – 2471Manganese; via tele nitrogenUniRule annotation
Binding sitei269 – 2691SubstrateUniRule annotation
Active sitei271 – 2711UniRule annotation
Metal bindingi296 – 2961ManganeseUniRule annotation
Binding sitei388 – 3881GTPUniRule annotation
Binding sitei419 – 4191GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi270 – 2756GTPUniRule annotation
Nucleotide bindingi512 – 5154GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-2280-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pckA
Ordered Locus Names:CT2232
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000001007 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103599Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT2232.

Structurei

3D structure databases

ProteinModelPortaliQ8KAD1.
SMRiQ8KAD1. Positions 12-600.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 3883Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8KAD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPIPINAPD SVRNLKLLQW VRETAELCQP DSVCWCDGSV EEYDRLCNEM
60 70 80 90 100
VASGTFIKLS EQKRPNSYLC RSDPSDVARV EDRTFICSIR RQDAGPTNNW
110 120 130 140 150
VAPKEMKATL NKLLAGCMKG RTMYIIPFSM GPLGSHIAHI GVEITDSPYV
160 170 180 190 200
VTNMRIMTRM GRAVLDLLDE EAEFVPCLHS VGAPLEPGQQ DVPWPCNDTK
210 220 230 240 250
YIVHFPEERS IVSYGSGYGG NALLGKKCFA LRIASSMARD EGWLAEHMLI
260 270 280 290 300
LGVESPEGEK DYVAAAFPSA CGKTNFAMMI PPGEMEGWKI TTVGDDIAWI
310 320 330 340 350
KQGKDGRLYA INPEYGFFGV APGTSEKSNP NAMATLHANC IFTNVALTPD
360 370 380 390 400
GDVWWEGMTD TPPDFLIDWQ GKPWVPGCER PAAHPNARFT APAHQCPVID
410 420 430 440 450
ENWENPDGVP ISAFIFGGRR GDTIPLVYQS ANWYYGVYLA ATMGSEKTAA
460 470 480 490 500
AAGKIGDVRR DPFAMLPFCG YHMGDYFNHW LHVGRTLTDP PRIFGVNWFR
510 520 530 540 550
KDENGKFLWP GFGENMRVLK WIIGRVHGRA AAVESPLGWM PRYESLDWRG
560 570 580 590 600
LDGFTRDKFS TLMSVDREAW KQELFSHEEL LEKLYDRLPK EFTHIRELML
610 620
STLWRSPEHW ELAPERYTAE H
Length:621
Mass (Da):69,933
Last modified:September 30, 2002 - v1
Checksum:i5F07BF2C8DA4C003
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM73448.1.
RefSeqiNP_663106.1. NC_002932.3.
WP_010933885.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM73448; AAM73448; CT2232.
GeneIDi1007328.
KEGGicte:CT2232.
PATRICi21402259. VBIChlTep116050_2025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM73448.1.
RefSeqiNP_663106.1. NC_002932.3.
WP_010933885.1. NC_002932.3.

3D structure databases

ProteinModelPortaliQ8KAD1.
SMRiQ8KAD1. Positions 12-600.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT2232.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM73448; AAM73448; CT2232.
GeneIDi1007328.
KEGGicte:CT2232.
PATRICi21402259. VBIChlTep116050_2025.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciCTEP194439:GHN0-2280-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / TLS.

Entry informationi

Entry nameiPCKG_CHLTE
AccessioniPrimary (citable) accession number: Q8KAD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2002
Last sequence update: September 30, 2002
Last modified: March 31, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.