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Protein

tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Gene

mnmG

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.By similarity

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei120FAD; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei366FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 13FAD1 Publication6
Nucleotide bindingi269 – 283NADSequence analysisAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processtRNA processing
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-2332-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Alternative name(s):
Glucose-inhibited division protein A
Gene namesi
Name:mnmG
Synonyms:gidA
Ordered Locus Names:CT2283
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170861 – 621tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmGAdd BLAST621

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits (By similarity).By similarity

Protein-protein interaction databases

STRINGi194439.CT2283

Structurei

Secondary structure

1621
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi11 – 22Combined sources12
Beta strandi27 – 32Combined sources6
Helixi34 – 36Combined sources3
Beta strandi44 – 47Combined sources4
Helixi49 – 61Combined sources13
Helixi65 – 72Combined sources8
Beta strandi73 – 79Combined sources7
Beta strandi81 – 83Combined sources3
Turni85 – 87Combined sources3
Beta strandi89 – 94Combined sources6
Helixi96 – 108Combined sources13
Beta strandi113 – 117Combined sources5
Beta strandi120 – 126Combined sources7
Beta strandi129 – 135Combined sources7
Beta strandi140 – 148Combined sources9
Beta strandi157 – 160Combined sources4
Beta strandi163 – 166Combined sources4
Beta strandi168 – 170Combined sources3
Helixi179 – 185Combined sources7
Beta strandi190 – 197Combined sources8
Beta strandi200 – 202Combined sources3
Helixi203 – 205Combined sources3
Turni208 – 210Combined sources3
Beta strandi211 – 214Combined sources4
Beta strandi223 – 226Combined sources4
Helixi231 – 233Combined sources3
Beta strandi238 – 242Combined sources5
Helixi245 – 252Combined sources8
Turni253 – 257Combined sources5
Helixi277 – 282Combined sources6
Beta strandi290 – 297Combined sources8
Beta strandi302 – 306Combined sources5
Helixi314 – 321Combined sources8
Beta strandi332 – 334Combined sources3
Beta strandi337 – 344Combined sources8
Helixi346 – 348Combined sources3
Beta strandi353 – 359Combined sources7
Beta strandi361 – 363Combined sources3
Helixi366 – 368Combined sources3
Helixi373 – 392Combined sources20
Turni401 – 403Combined sources3
Helixi405 – 415Combined sources11
Turni424 – 426Combined sources3
Helixi433 – 435Combined sources3
Helixi437 – 439Combined sources3
Helixi440 – 450Combined sources11
Helixi456 – 478Combined sources23
Helixi483 – 493Combined sources11
Helixi505 – 508Combined sources4
Beta strandi509 – 512Combined sources4
Helixi515 – 519Combined sources5
Helixi523 – 528Combined sources6
Helixi531 – 534Combined sources4
Helixi536 – 547Combined sources12
Helixi549 – 565Combined sources17
Beta strandi572 – 574Combined sources3
Helixi586 – 593Combined sources8
Helixi598 – 601Combined sources4
Helixi609 – 616Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CP8X-ray3.20A/B/C/D1-621[»]
ProteinModelPortaliQ8KA85
SMRiQ8KA85
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8KA85

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family.Curated

Phylogenomic databases

eggNOGiENOG4105CWE Bacteria
COG0445 LUCA
HOGENOMiHOG000201060
KOiK03495
OMAiFRPGYAI
OrthoDBiPOG091H01O0

Family and domain databases

Gene3Di3.50.50.60, 2 hits
HAMAPiMF_00129 MnmG_GidA, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR026904 GidA-assoc_3
IPR004416 MnmG
IPR002218 MnmG-rel
IPR020595 MnmG-rel_CS
PANTHERiPTHR11806 PTHR11806, 1 hit
PTHR11806:SF0 PTHR11806:SF0, 1 hit
PfamiView protein in Pfam
PF01134 GIDA, 1 hit
PF13932 GIDA_assoc, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
TIGRFAMsiTIGR00136 gidA, 1 hit
PROSITEiView protein in PROSITE
PS01280 GIDA_1, 1 hit
PS01281 GIDA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8KA85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDVIVVGAG HAGCEAALAV ARGGLHCLLI TSDLSAVARM SCNPAIGGVA
60 70 80 90 100
KGQITREIDA LGGEMGKAID ATGIQFRMLN RSKGPAMHSP RAQADKTQYS
110 120 130 140 150
LYMRRIVEHE PNIDLLQDTV IGVSANSGKF SSVTVRSGRA IQAKAAILAC
160 170 180 190 200
GTFLNGLIHI GMDHFPGGRS TAEPPVEGLT ESLASLGFSF GRLKTGTPPR
210 220 230 240 250
IDSRSVDYTI VTEQPGDVDP VPFSFSSTSV ANRNLVSCYL TKTTEKTHDI
260 270 280 290 300
LRTGFDRSPL FTGKVQGVGP RYCPSIEDKI SRFPDKSSHH IFLEPEGTDT
310 320 330 340 350
VEMYVNGFST SLPEDIQIAG LRSIPGLEEA KMIRPGYAIE YDFFHPWQIR
360 370 380 390 400
STMETRPVEN LFFAGQINGT SGYEEAAAQG LMAGINAVRK ILGKELIVLG
410 420 430 440 450
RDQAYIGVLI DDLITKETKE PYRMFTSSAE HRLILRHDNA DLRLRKIGYD
460 470 480 490 500
CNLVSSDDLH RTESIIKRVQ HCLEVMKTAK VTPAEINTLL MNKGLQELKT
510 520 530 540 550
PARALSLIKR PGISLQDILE HSLSVRSAAE ELCNDPRVAE QVQIEIKYEG
560 570 580 590 600
YIKREQLVAD RIARLDSLHI PDNFNYDSLN SLSSEGREKL LKHRPATIGQ
610 620
ASRILGVSPS DVSILMIRLG R
Length:621
Mass (Da):68,097
Last modified:October 1, 2002 - v1
Checksum:i752ED5CA7F9A38BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA Translation: AAM73496.1
RefSeqiNP_663154.1, NC_002932.3
WP_010933931.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM73496; AAM73496; CT2283
GeneIDi1007276
KEGGicte:CT2283
PATRICifig|194439.7.peg.2076

Similar proteinsi

Entry informationi

Entry nameiMNMG_CHLTE
AccessioniPrimary (citable) accession number: Q8KA85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health