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Protein

tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Gene

mnmG

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.By similarity

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei120FAD; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei366FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 13FAD1 Publication6
Nucleotide bindingi269 – 283NADSequence analysisAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Alternative name(s):
Glucose-inhibited division protein A
Gene namesi
Name:mnmG
Synonyms:gidA
Ordered Locus Names:CT2283
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170861 – 621tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmGAdd BLAST621

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits (By similarity).By similarity

Protein-protein interaction databases

STRINGi194439.CT2283.

Structurei

Secondary structure

1621
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi11 – 22Combined sources12
Beta strandi27 – 32Combined sources6
Helixi34 – 36Combined sources3
Beta strandi44 – 47Combined sources4
Helixi49 – 61Combined sources13
Helixi65 – 72Combined sources8
Beta strandi73 – 79Combined sources7
Beta strandi81 – 83Combined sources3
Turni85 – 87Combined sources3
Beta strandi89 – 94Combined sources6
Helixi96 – 108Combined sources13
Beta strandi113 – 117Combined sources5
Beta strandi120 – 126Combined sources7
Beta strandi129 – 135Combined sources7
Beta strandi140 – 148Combined sources9
Beta strandi157 – 160Combined sources4
Beta strandi163 – 166Combined sources4
Beta strandi168 – 170Combined sources3
Helixi179 – 185Combined sources7
Beta strandi190 – 197Combined sources8
Beta strandi200 – 202Combined sources3
Helixi203 – 205Combined sources3
Turni208 – 210Combined sources3
Beta strandi211 – 214Combined sources4
Beta strandi223 – 226Combined sources4
Helixi231 – 233Combined sources3
Beta strandi238 – 242Combined sources5
Helixi245 – 252Combined sources8
Turni253 – 257Combined sources5
Helixi277 – 282Combined sources6
Beta strandi290 – 297Combined sources8
Beta strandi302 – 306Combined sources5
Helixi314 – 321Combined sources8
Beta strandi332 – 334Combined sources3
Beta strandi337 – 344Combined sources8
Helixi346 – 348Combined sources3
Beta strandi353 – 359Combined sources7
Beta strandi361 – 363Combined sources3
Helixi366 – 368Combined sources3
Helixi373 – 392Combined sources20
Turni401 – 403Combined sources3
Helixi405 – 415Combined sources11
Turni424 – 426Combined sources3
Helixi433 – 435Combined sources3
Helixi437 – 439Combined sources3
Helixi440 – 450Combined sources11
Helixi456 – 478Combined sources23
Helixi483 – 493Combined sources11
Helixi505 – 508Combined sources4
Beta strandi509 – 512Combined sources4
Helixi515 – 519Combined sources5
Helixi523 – 528Combined sources6
Helixi531 – 534Combined sources4
Helixi536 – 547Combined sources12
Helixi549 – 565Combined sources17
Beta strandi572 – 574Combined sources3
Helixi586 – 593Combined sources8
Helixi598 – 601Combined sources4
Helixi609 – 616Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CP8X-ray3.20A/B/C/D1-621[»]
ProteinModelPortaliQ8KA85.
SMRiQ8KA85.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8KA85.

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family.Curated

Phylogenomic databases

eggNOGiENOG4105CWE. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201060.
KOiK03495.
OMAiFRPGYAI.
OrthoDBiPOG091H01O0.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8KA85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDVIVVGAG HAGCEAALAV ARGGLHCLLI TSDLSAVARM SCNPAIGGVA
60 70 80 90 100
KGQITREIDA LGGEMGKAID ATGIQFRMLN RSKGPAMHSP RAQADKTQYS
110 120 130 140 150
LYMRRIVEHE PNIDLLQDTV IGVSANSGKF SSVTVRSGRA IQAKAAILAC
160 170 180 190 200
GTFLNGLIHI GMDHFPGGRS TAEPPVEGLT ESLASLGFSF GRLKTGTPPR
210 220 230 240 250
IDSRSVDYTI VTEQPGDVDP VPFSFSSTSV ANRNLVSCYL TKTTEKTHDI
260 270 280 290 300
LRTGFDRSPL FTGKVQGVGP RYCPSIEDKI SRFPDKSSHH IFLEPEGTDT
310 320 330 340 350
VEMYVNGFST SLPEDIQIAG LRSIPGLEEA KMIRPGYAIE YDFFHPWQIR
360 370 380 390 400
STMETRPVEN LFFAGQINGT SGYEEAAAQG LMAGINAVRK ILGKELIVLG
410 420 430 440 450
RDQAYIGVLI DDLITKETKE PYRMFTSSAE HRLILRHDNA DLRLRKIGYD
460 470 480 490 500
CNLVSSDDLH RTESIIKRVQ HCLEVMKTAK VTPAEINTLL MNKGLQELKT
510 520 530 540 550
PARALSLIKR PGISLQDILE HSLSVRSAAE ELCNDPRVAE QVQIEIKYEG
560 570 580 590 600
YIKREQLVAD RIARLDSLHI PDNFNYDSLN SLSSEGREKL LKHRPATIGQ
610 620
ASRILGVSPS DVSILMIRLG R
Length:621
Mass (Da):68,097
Last modified:October 1, 2002 - v1
Checksum:i752ED5CA7F9A38BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM73496.1.
RefSeqiNP_663154.1. NC_002932.3.
WP_010933931.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM73496; AAM73496; CT2283.
GeneIDi1007276.
KEGGicte:CT2283.
PATRICi21402363. VBIChlTep116050_2076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM73496.1.
RefSeqiNP_663154.1. NC_002932.3.
WP_010933931.1. NC_002932.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CP8X-ray3.20A/B/C/D1-621[»]
ProteinModelPortaliQ8KA85.
SMRiQ8KA85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT2283.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM73496; AAM73496; CT2283.
GeneIDi1007276.
KEGGicte:CT2283.
PATRICi21402363. VBIChlTep116050_2076.

Phylogenomic databases

eggNOGiENOG4105CWE. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201060.
KOiK03495.
OMAiFRPGYAI.
OrthoDBiPOG091H01O0.

Miscellaneous databases

EvolutionaryTraceiQ8KA85.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMNMG_CHLTE
AccessioniPrimary (citable) accession number: Q8KA85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.