Reviewed, UniProtKB/Swiss-Prot Q8KA76 (RP32_BUCAP)

Names and origin

Protein names

Recommended name:
    RNA polymerase sigma-32 factor

Gene names

Name:	rpoH
Ordered Locus Names:	BUsg_026

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters By similarity.
Sequence similarities	Belongs to the sigma-70 factor family. Sigma-32 subfamily.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to heat Inferred from electronic annotation. Source: InterPro transcription initiation Inferred from electronic annotation. Source: InterPro
Molecular function	sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	RNA polymerase sigma-32 factor		PRO_0000093952
Regions
	DNA binding	254 – 273	20	H-T-H motif By similarity
	Motif	77 – 90	14	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8KA76-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: FA73D1A5D725250F	FASTA	285	33,298
	10 20 30 40 50 60 MTNKVQILSV TALGNLDAYI RIANLWPMLS IKEEKLLTKR LRYHDDLDAA KTLILSHLRF 70 80 90 100 110 120 VIHISRNYSG YGLLQADLIQ EGNIGLMKAV RRFNPDIGVR LVSFAVHWIK SEIHEYVLRN 130 140 150 160 170 180 WRIVKVATTK SQRKLFFNLR KNKKRLGWFN QEEIEIVARE LGVSSEDVRE MESRMSAQDI 190 200 210 220 230 240 TFNPFPEEDL KDGKINGNMF YLQDKTSNFA NGLEQDNWNK HTTSKLSNAL LRLDERSRNI 250 260 270 280 IRARWLDKKE KNTLQKIANN YGISAERVRQ LEKNAMKKLK IAIEN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67597.1.
RefSeq	NP_660386.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005842.
GenomeReviews	Gene locus BUsg_026 in contig AE013218_GR.
KEGG	bas:BUsg026.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8KA76.
OMA	SAQDITF
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG026-MON.
Family and domain databases
InterPro	IPR014284. RNA_pol_sigma-70. IPR000943. RNA_pol_sigma70. IPR007627. RNA_pol_sigma70_r2. IPR007630. RNA_pol_sigma70_r4. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012759. RNA_pol_sigma_RpoH_proteobac. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF04542. Sigma70_r2. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
TIGRFAMs	TIGR02392. rpoH_proteo. 1 hit. TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RP32_BUCAP

Accession

Primary (citable) accession number: Q8KA76

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	November 24, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)