Q8KA75 (GLMS_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]
EC=2.6.1.16

Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine-D-fructose-6-phosphate amidotransferase

Gene names

Name:	glmS
Ordered Locus Names:	BUsg_027

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity. HAMAP MF_00164
Catalytic activity	L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate. HAMAP MF_00164
Subunit structure	Homodimer By similarity. HAMAP MF_00164
Subcellular location	Cytoplasm By similarity HAMAP MF_00164.
Sequence similarities	Contains 1 glutamine amidotransferase type-2 domain. Contains 2 SIS domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase Repeat
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 608	607	Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] HAMAP MF_00164		PRO_0000135312
Regions
	Domain	2 – 219	218	Glutamine amidotransferase type-2
	Domain	287 – 427	141	SIS 1
	Domain	459 – 598	140	SIS 2
Sites
	Active site	2	1	Nucleophile; for GATase activity By similarity
	Active site	603	1	For Fru-6P isomerization activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA75 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 508A3C69D37BAB86	FASTA	608	68,477
	10 20 30 40 50 60 MCGIVAAVTQ RNIIDFLLEG IKKLEYRGYD SSGLAIINKN QNIVRIRCVG RVNKLLKKVK 70 80 90 100 110 120 KKKLFSNIGL AHTRWATHGQ VSEKNTHPHI SSHIAVVHNG VIENNTKLRH FLEKKGYIFY 130 140 150 160 170 180 SDTDTEVIAH LLHWEQTKTG ESLVEVIQRS KMKLNGNYSM VVMDAYNSSR LIAVRSGSPL 190 200 210 220 230 240 VIGLGIKENF IASDQIALLN ITKRFIYLEE GDTAIVETKK INIFNKNNLI IKRKEITSNV 250 260 270 280 290 300 KYKSINKGKY KHYMEKEIYE QPISIQNTLN NRIKKDTINF SELGDREKKF FLNAEHIQIV 310 320 330 340 350 360 ACGTSYHAAM VSRYWFESLA NIPCDVEIAS EFSSRKLAVR KKSVFITLSQ SGETADTLAA 370 380 390 400 410 420 LRISKKLGYL GSLTICNMKG SSLVQESDCY LLTKAGIEIG VASTKSFTTQ LTVLLMLIAK 430 440 450 460 470 480 ITKLKKQKNN IEKKIAQILS LLPSKVEAIL KKNTLIKTLA NQLCNKKNIL FLGRGDQYPI 490 500 510 520 530 540 AMEGALKLKE ISYIHAEAYP SGELKHGPLA LIDKNIPVII TAPKNSLLEK TKKNIKEICA 550 560 570 580 590 600 RGGIIYVFSD QEIDLEKNMT IIKIPYVEEI IAPILYIIPL QLLAYYIALS KGKNIDQPRH LAKSVTVE

References

[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67598.1.
RefSeq	NP_660387.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8KA75.
SMR	Q8KA75. Positions 2-608.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000306; EBBUCP00000000306; EBBUCG00000000306.
GeneID	1005843.
GenomeReviews	Gene locus BUsg_027 in contig AE013218_GR.
KEGG	bas:BUsg027.
PATRIC	21246811. VBIBucAph100086_0027.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007826.
HOGENOM	HBG645312.
OMA	YNNISSH.
ProtClustDB	PRK00331.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG027-MONOMER.
Family and domain databases
HAMAP	MF_00164. GlmS. [Tree]
InterPro	IPR000583. GATase_2. IPR017932. GATase_II. IPR005855. GlmS_trans. IPR001347. SIS. [Graphical view]
KO	K00820.
Pfam	PF00310. GATase_2. 1 hit. PF01380. SIS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01135. GlmS. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS51464. SIS. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLMS_BUCAP

Accession

Primary (citable) accession number: Q8KA75

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 63 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program