Reviewed,
UniProtKB/Swiss-Prot Q8KA74 (GLMU_BUCAP)
Last modified
June 16, 2009.
Version 49.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (3) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Bifunctional protein glmU Including the following 2 domains: 1- Recommended name: UDP-N-acetylglucosamine pyrophosphorylase EC=2.7.7.23 Alternative name(s): N-acetylglucosamine-1-phosphate uridyltransferase 2- Recommended name: Glucosamine-1-phosphate N-acetyltransferase EC=2.3.1.157 | ||||
| Gene names |
| ||||
| Organism | Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 98794 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera |
Protein attributes
| Sequence length | 461 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc By similarity. |
| Catalytic activity | Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate. HAMAP MF_01631 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-D-glucosamine. HAMAP MF_01631 |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. |
| Pathway | Nucleotide-sugar biosynthesis; UDP-N-acetyl-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from D-glucosamine 6-phosphate (route II): step 2/2. HAMAP MF_01631 Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_01631 |
| Subunit structure | Homotrimer By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family. In the C-terminal section; belongs to the transferase hexapeptide repeat family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 461 | 461 | Bifunctional protein glmU HAMAP MF_01631 | PRO_0000068699 | |||||
Regions | |||||||||
| Region | 1 – 229 | 229 | Pyrophosphorylase By similarity | ||||||
| Region | 11 – 14 | 4 | Substrate binding By similarity | ||||||
| Region | 81 – 82 | 2 | Substrate binding By similarity | ||||||
| Region | 230 – 250 | 21 | Linker By similarity | ||||||
| Region | 251 – 461 | 211 | N-acetyltransferase By similarity | ||||||
Sites | |||||||||
| Active site | 363 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 105 | 1 | Magnesium By similarity | ||||||
| Metal binding | 227 | 1 | Magnesium By similarity | ||||||
| Binding site | 76 | 1 | Substrate By similarity | ||||||
| Binding site | 140 | 1 | Substrate; via amide nitrogen By similarity | ||||||
| Binding site | 154 | 1 | Substrate By similarity | ||||||
| Binding site | 387 | 1 | Acetyl-CoA By similarity | ||||||
| Binding site | 423 | 1 | Acetyl-CoA; via amide nitrogen By similarity | ||||||
Sequences
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References
| [1] | "50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| AE013218 Genomic DNA. Translation: AAM67599.1. | |
| RefSeq | NP_660388.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1HV9 based on UniProtKB P17114. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1005844. |
| GenomeReviews | Gene locus BUsg_028 in contig AE013218_GR. |
| KEGG | bas:BUsg028. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q8KA74. |
| OMA | Q8KA74. TRMKSKL. |
Enzyme and pathway databases | |
| BioCyc | BAPH198804:BUSG028-MON. |
Family and domain databases | |
| HAMAP | MF_01631. [Tree] |
| InterPro | IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR001228. ISPD_synthase. IPR005882. UDP_GlcNAc_PyrPase. [Graphical view] |
| Pfam | PF00132. Hexapep. 5 hits. PF01128. IspD. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01173. glmU. 1 hit. |
| PROSITE | PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GLMU_BUCAP | ||||||||
| Accession | Primary (citable) accession number: Q8KA74 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Buchnera aphidicola (subsp. Schizaphis graminum) Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
