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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.UniRule annotation

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi640 – 6401ZincUniRule annotation
Metal bindingi642 – 6421ZincUniRule annotation
Metal bindingi725 – 7251ZincUniRule annotation

GO - Molecular functioni

  1. 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Source: UniProtKB-HAMAP
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-32-MONOMER.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseUniRule annotation (EC:2.1.1.14UniRule annotation)
Alternative name(s):
Cobalamin-independent methionine synthaseUniRule annotation
Methionine synthase, vitamin-B12 independent isozymeUniRule annotation
Gene namesi
Name:metEUniRule annotation
Ordered Locus Names:BUsg_031
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7577575-methyltetrahydropteroyltriglutamate--homocysteine methyltransferasePRO_0000098620Add
BLAST

Proteomic databases

PRIDEiQ8KA71.

Interactioni

Protein-protein interaction databases

STRINGi198804.BUsg031.

Structurei

3D structure databases

ProteinModelPortaliQ8KA71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the vitamin-B12 independent methionine synthase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0620.
KOiK00549.
OMAiFTKFGWV.
OrthoDBiEOG6FFS3G.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KA71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIFNHTLGF PRIGLNRELK KAQEDYWSGN LLYEDFLCVG SQLRRENWKK
60 70 80 90 100
QKDAGIDYIP VGDFAWYDHV LSTSMMLGNI PERHLNVNDN KIDLDCLFRI
110 120 130 140 150
ARGCSPDVAA SEMTKWFNTN YHYIVPEFNK NRILKFSWKQ ILEETDEALL
160 170 180 190 200
LGYKVKPIIL GPITYLWLGK IKGEYFDRLD LLKNILPIYK QVLRELSQRN
210 220 230 240 250
IDFVQIDEPA LVLDLPEKWK KAYSYAYEYL NGTTKILLTT YFDSIEHNIE
260 270 280 290 300
YIRDLPIYGI HIDLVFGKYN LFDFNSKLPK EWILSLGVIN GRNVWRADLI
310 320 330 340 350
KLFKSISTIL KFRKKLLIGS SCSLLHSPID LQKEKNLDKE SKKWFSFAVQ
360 370 380 390 400
KCTELSLLSK ALNKNDTDSI QKWCSSIYER NFSKRVQKIE VQNRLSEILN
410 420 430 440 450
VNFKRSDSYN IRSREQKKKF NLPILPTTTI GSFPQTLQIR KLRRDYKQGL
460 470 480 490 500
INDVDYEIGI KKHIKEVIKE QEKLGIDVLV HGEPERNDMV EYFGENLDGF
510 520 530 540 550
IFTDNGWVQS YGSRCVKPPI IIGDVSRLKP ITVKWSKYAQ SLTDKPVKAM
560 570 580 590 600
LTGPVTILFW SFPREDISLE KIAKQIGLAL RDEVLDLEKE KIEIIQIDEP
610 620 630 640 650
ALREGLPLRK SLWNEYLSWA VDAFRLSSSG VKNTTQIHTH MCYCEFHDIM
660 670 680 690 700
HAISFLDADV ITIETARSDM ELLKSFKTFK YPNEIGPGVY DIHSSNIPSI
710 720 730 740 750
TSIKFLLNKA IAYIPVERIW VNPDCGLKTR NWNETILALK NMVCAAKEMR

DKIKKIT
Length:757
Mass (Da):88,098
Last modified:October 1, 2002 - v1
Checksum:i637B1D9532325BCE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67602.1.
RefSeqiNP_660391.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67602; AAM67602; BUsg_031.
KEGGibas:BUsg031.
PATRICi21246819. VBIBucAph100086_0031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67602.1.
RefSeqiNP_660391.1. NC_004061.1.

3D structure databases

ProteinModelPortaliQ8KA71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg031.

Proteomic databases

PRIDEiQ8KA71.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM67602; AAM67602; BUsg_031.
KEGGibas:BUsg031.
PATRICi21246819. VBIBucAph100086_0031.

Phylogenomic databases

eggNOGiCOG0620.
KOiK00549.
OMAiFTKFGWV.
OrthoDBiEOG6FFS3G.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BioCyciBAPH198804:GHMG-32-MONOMER.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiMETE_BUCAP
AccessioniPrimary (citable) accession number: Q8KA71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: April 29, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.