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Q8KA71 (METE_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

EC=2.1.1.14
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene names
Name:metE
Ordered Locus Names:BUsg_031
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP-Rule MF_00172

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP-Rule MF_00172

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP-Rule MF_00172

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7577575-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP-Rule MF_00172
PRO_0000098620

Sites

Metal binding6401Zinc By similarity
Metal binding6421Zinc By similarity
Metal binding7251Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8KA71 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 637B1D9532325BCE

FASTA75788,098
        10         20         30         40         50         60 
MTIFNHTLGF PRIGLNRELK KAQEDYWSGN LLYEDFLCVG SQLRRENWKK QKDAGIDYIP 

        70         80         90        100        110        120 
VGDFAWYDHV LSTSMMLGNI PERHLNVNDN KIDLDCLFRI ARGCSPDVAA SEMTKWFNTN 

       130        140        150        160        170        180 
YHYIVPEFNK NRILKFSWKQ ILEETDEALL LGYKVKPIIL GPITYLWLGK IKGEYFDRLD 

       190        200        210        220        230        240 
LLKNILPIYK QVLRELSQRN IDFVQIDEPA LVLDLPEKWK KAYSYAYEYL NGTTKILLTT 

       250        260        270        280        290        300 
YFDSIEHNIE YIRDLPIYGI HIDLVFGKYN LFDFNSKLPK EWILSLGVIN GRNVWRADLI 

       310        320        330        340        350        360 
KLFKSISTIL KFRKKLLIGS SCSLLHSPID LQKEKNLDKE SKKWFSFAVQ KCTELSLLSK 

       370        380        390        400        410        420 
ALNKNDTDSI QKWCSSIYER NFSKRVQKIE VQNRLSEILN VNFKRSDSYN IRSREQKKKF 

       430        440        450        460        470        480 
NLPILPTTTI GSFPQTLQIR KLRRDYKQGL INDVDYEIGI KKHIKEVIKE QEKLGIDVLV 

       490        500        510        520        530        540 
HGEPERNDMV EYFGENLDGF IFTDNGWVQS YGSRCVKPPI IIGDVSRLKP ITVKWSKYAQ 

       550        560        570        580        590        600 
SLTDKPVKAM LTGPVTILFW SFPREDISLE KIAKQIGLAL RDEVLDLEKE KIEIIQIDEP 

       610        620        630        640        650        660 
ALREGLPLRK SLWNEYLSWA VDAFRLSSSG VKNTTQIHTH MCYCEFHDIM HAISFLDADV 

       670        680        690        700        710        720 
ITIETARSDM ELLKSFKTFK YPNEIGPGVY DIHSSNIPSI TSIKFLLNKA IAYIPVERIW 

       730        740        750 
VNPDCGLKTR NWNETILALK NMVCAAKEMR DKIKKIT 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67602.1.
RefSeqNP_660391.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8KA71.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg031.

Proteomic databases

PRIDEQ8KA71.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67602; AAM67602; BUsg_031.
GeneID1005847.
KEGGbas:BUsg031.
PATRIC21246819. VBIBucAph100086_0031.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0620.
KOK00549.
OMASFPQTSE.
OrthoDBEOG6FFS3G.
ProtClustDBPRK05222.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-32-MONOMER.
UniPathwayUPA00051; UER00082.

Family and domain databases

HAMAPMF_00172. Meth_synth.
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETE_BUCAP
AccessionPrimary (citable) accession number: Q8KA71
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names