Q8KA70 (PUR9_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	purH
Ordered Locus Names:	BUsg_032

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139
Sequence similarities	Belongs to the PurH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139		PRO_0000192077

Sequences

	Sequence		Length	Mass (Da)
	Q8KA70 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 7F0F13954EF902A5	FASTA	526	59,422
	10 20 30 40 50 60 MSLNNIIKNA LVSLSDKTDL LKISKILAEK KINLFCTGGT ADVLKKNKIP VLEISDYTNF 70 80 90 100 110 120 PEIMNGRLKT LHPKIIGGIL GRREKDQKIM KLHNLIPIDI VIVNFYPFEK IQNRKEFTIE 130 140 150 160 170 180 EIIDNIDIGG PTLVRAAAKN YKDVIVIVDL SDFTSCIQLI NTNTVSLETR FDLATKAFKY 190 200 210 220 230 240 TALYEEIISK YFLKKNPYRK KHQKNIFPNE FQLNFIKKQD LRYGENQHQK SSFYIEKEIL 250 260 270 280 290 300 KSGTISSSNQ IQGKNLSYNN ICDADIALEC VKEFSKPTCV IVKHGNPCGV SESNSLIKAY 310 320 330 340 350 360 FSAYNADPIS AFGGIIAFNC LLDLDTAQEI VKKQFVEVII APEIDEMAVK ILKRKKNIRL 370 380 390 400 410 420 LICGKIEKNK KGLDFKRITN GLLIQEYDCD EINAKNFDFV TNRLPTEKEL EDAIFSWKVA 430 440 450 460 470 480 KFVKSNAIVY SLNKTTIGIG AGQTSRIDAT KLANLKVKDR NHNNTTGATM ASDAFFPFRD 490 500 510 520 GIDNAALIGI SCIIQPGGSI RDKEVIESAN EHNISMIFTK KRHFKH

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67603.1.
RefSeq	NP_660392.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8KA70.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg032.
Proteomic databases
PRIDE	Q8KA70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67603; AAM67603; BUsg_032.
GeneID	1005848.
KEGG	bas:BUsg032.
PATRIC	21246821. VBIBucAph100086_0032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0138.
KO	K00602.
OMA	DLLFAWK.
ProtClustDB	PRK00881.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-104-MONOMER.
UniPathway	UPA00074; UER00133. UPA00074; UER00135.
Family and domain databases
Gene3D	3.40.140.20. 2 hits. 3.40.50.1380. 1 hit.
HAMAP	MF_00139. PurH.
InterPro	IPR024051. AICAR_Tfase_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
PANTHER	PTHR11692. PTHR11692. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BUCAP

Accession

Primary (citable) accession number: Q8KA70

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program