Q8KA63 (MURB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BUsg_042

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179188
Regions
	Domain	19 – 189	171	FAD-binding PCMH-type
Sites
	Active site	165	1	By similarity
	Active site	235	1	Proton donor By similarity
	Active site	331	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA63 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 4D3E909931F725CB	FASTA	344	39,519
	10 20 30 40 50 60 MYKKKKSYQS LKNLNTFSIN VTAKKIIFVK TIQSLMKIWK TCNLSNIPYI ILGEGSNVLF 70 80 90 100 110 120 LENYAGVVII NRIKGIRIEE KKKNWLLHVF SGEKWHDLVK YTLRMGFFGL ENLALIPGSV 130 140 150 160 170 180 GSAAIQNIGA YGLELKNICQ YVDVISLENG KTIRLKKKTC NFSYRSSIFK YKYNNGYAVI 190 200 210 220 230 240 AVGIKIKKNW KPVIFSSLLK SKKILEINAY KIFNIVCQIR KKKLPNLKKL GNAGSFFKNP 250 260 270 280 290 300 IITSKKTKKI LSSYMKMPYY IQKNGFIKIP AAWLIEKYNF KNIQIGDAAI YKKQKLILIN 310 320 330 340 LKKANSKDIL KLAQIIQKCI LKKFGIYLEP EVDFINSLGK VKLL

References

[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67613.1.
RefSeq	NP_660402.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8KA63.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000119; EBBUCP00000000119; EBBUCG00000000119.
GeneID	1005858.
GenomeReviews	Gene locus BUsg_042 in contig AE013218_GR.
KEGG	bas:BUsg042.
PATRIC	21246849. VBIBucAph100086_0042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007998.
HOGENOM	HBG686573.
OMA	IEQAGWK.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG042-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BUCAP

Accession

Primary (citable) accession number: Q8KA63

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program