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Q8KA62

- METF_BUCAP

UniProt

Q8KA62 - METF_BUCAP

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Protein
5,10-methylenetetrahydrofolate reductase
Gene
metF, BUsg_043
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptor By similarity
Binding sitei88 – 881FAD By similarity
Binding sitei120 – 1201Substrate By similarity
Binding sitei152 – 1521FAD By similarity
Binding sitei183 – 1831Substrate By similarity
Binding sitei219 – 2191Substrate By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei275 – 2751Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NAD By similarity
Nucleotide bindingi59 – 624FAD By similarity
Nucleotide bindingi59 – 602NAD By similarity
Nucleotide bindingi118 – 1203FAD By similarity
Nucleotide bindingi131 – 1322FAD By similarity
Nucleotide bindingi156 – 1594FAD By similarity
Nucleotide bindingi165 – 1728FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-48-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:BUsg_043
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2922925,10-methylenetetrahydrofolate reductase
PRO_0000190259Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi198804.BUsg043.

Structurei

3D structure databases

ProteinModelPortaliQ8KA62.
SMRiQ8KA62. Positions 22-292.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
KOiK00297.
OMAiHENGSSH.
OrthoDBiEOG6D2KVW.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KA62-1 [UniParc]FASTAAdd to Basket

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MNFLHQYYQD IIKKKLENLN DKIQCSFEFF PPKNLDTEKK LFSSVVKLSL    50
LKPFLFSVTY GANSGERKKT YSIVQRIHKK TGITTAPHLT CVDASLSELK 100
EIAKFYWENG IRSIVALRGD VPNKSYQHKI YALDLVVLLK KIADFDISVA 150
AYPEIHPESK NAQSDILNLK KKADAGANRA ITQFFFNVES YLRFRDNCIK 200
NNINIEIIPG ILPIYNFEQL KKFSSMTNVS IPKWMFEMFN GLDEDLETQK 250
IIGSNIVINI VKTLLYEGVK NFHFYTLNKS DVIYSICHMF GL 292
Length:292
Mass (Da):33,676
Last modified:October 1, 2002 - v1
Checksum:i0660895E1B065E71
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67614.1.
RefSeqiNP_660403.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67614; AAM67614; BUsg_043.
GeneIDi1005859.
KEGGibas:BUsg043.
PATRICi21246851. VBIBucAph100086_0043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67614.1 .
RefSeqi NP_660403.1. NC_004061.1.

3D structure databases

ProteinModelPortali Q8KA62.
SMRi Q8KA62. Positions 22-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198804.BUsg043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM67614 ; AAM67614 ; BUsg_043 .
GeneIDi 1005859.
KEGGi bas:BUsg043.
PATRICi 21246851. VBIBucAph100086_0043.

Phylogenomic databases

eggNOGi COG0685.
KOi K00297.
OMAi HENGSSH.
OrthoDBi EOG6D2KVW.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci BAPH198804:GHMG-48-MONOMER.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiMETF_BUCAP
AccessioniPrimary (citable) accession number: Q8KA62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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