Reviewed, UniProtKB/Swiss-Prot Q8KA62 (METF_BUCAP)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	BUsg_043

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	5,10-methylenetetrahydrofolate reductase		PRO_0000190259

Sequences

	Sequence		Length	Mass (Da)
	Q8KA62-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0660895E1B065E71	FASTA	292	33,676
	10 20 30 40 50 60 MNFLHQYYQD IIKKKLENLN DKIQCSFEFF PPKNLDTEKK LFSSVVKLSL LKPFLFSVTY 70 80 90 100 110 120 GANSGERKKT YSIVQRIHKK TGITTAPHLT CVDASLSELK EIAKFYWENG IRSIVALRGD 130 140 150 160 170 180 VPNKSYQHKI YALDLVVLLK KIADFDISVA AYPEIHPESK NAQSDILNLK KKADAGANRA 190 200 210 220 230 240 ITQFFFNVES YLRFRDNCIK NNINIEIIPG ILPIYNFEQL KKFSSMTNVS IPKWMFEMFN 250 260 270 280 290 GLDEDLETQK IIGSNIVINI VKTLLYEGVK NFHFYTLNKS DVIYSICHMF GL

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67614.1.
RefSeq	NP_660403.1.
3D structure databases
SMR	Q8KA62. Positions 22-292.
ModBase	Search...
Genome annotation databases
GeneID	1005859.
GenomeReviews	Gene locus BUsg_043 in contig AE013218_GR.
KEGG	bas:BUsg043.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649727.
OMA	FFPPKTP.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG043-MONOMER.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_BUCAP

Accession

Primary (citable) accession number: Q8KA62

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)