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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptorBy similarity
Binding sitei88 – 881FADBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei152 – 1521FADBy similarity
Binding sitei183 – 1831SubstrateBy similarity
Binding sitei219 – 2191SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei275 – 2751SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NADBy similarity
Nucleotide bindingi59 – 624FADBy similarity
Nucleotide bindingi59 – 602NADBy similarity
Nucleotide bindingi118 – 1203FADBy similarity
Nucleotide bindingi131 – 1322FADBy similarity
Nucleotide bindingi156 – 1594FADBy similarity
Nucleotide bindingi165 – 1728FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-48-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:BUsg_043
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2922925,10-methylenetetrahydrofolate reductasePRO_0000190259Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi198804.BUsg043.

Structurei

3D structure databases

ProteinModelPortaliQ8KA62.
SMRiQ8KA62. Positions 22-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
KOiK00297.
OMAiFIRAETG.
OrthoDBiEOG6D2KVW.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8KA62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFLHQYYQD IIKKKLENLN DKIQCSFEFF PPKNLDTEKK LFSSVVKLSL
60 70 80 90 100
LKPFLFSVTY GANSGERKKT YSIVQRIHKK TGITTAPHLT CVDASLSELK
110 120 130 140 150
EIAKFYWENG IRSIVALRGD VPNKSYQHKI YALDLVVLLK KIADFDISVA
160 170 180 190 200
AYPEIHPESK NAQSDILNLK KKADAGANRA ITQFFFNVES YLRFRDNCIK
210 220 230 240 250
NNINIEIIPG ILPIYNFEQL KKFSSMTNVS IPKWMFEMFN GLDEDLETQK
260 270 280 290
IIGSNIVINI VKTLLYEGVK NFHFYTLNKS DVIYSICHMF GL
Length:292
Mass (Da):33,676
Last modified:October 1, 2002 - v1
Checksum:i0660895E1B065E71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67614.1.
RefSeqiNP_660403.1. NC_004061.1.
WP_011053580.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67614; AAM67614; BUsg_043.
KEGGibas:BUsg043.
PATRICi21246851. VBIBucAph100086_0043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67614.1.
RefSeqiNP_660403.1. NC_004061.1.
WP_011053580.1. NC_004061.1.

3D structure databases

ProteinModelPortaliQ8KA62.
SMRiQ8KA62. Positions 22-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM67614; AAM67614; BUsg_043.
KEGGibas:BUsg043.
PATRICi21246851. VBIBucAph100086_0043.

Phylogenomic databases

eggNOGiCOG0685.
KOiK00297.
OMAiFIRAETG.
OrthoDBiEOG6D2KVW.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciBAPH198804:GHMG-48-MONOMER.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiMETF_BUCAP
AccessioniPrimary (citable) accession number: Q8KA62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: April 29, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.