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Q8KA62

- METF_BUCAP

UniProt

Q8KA62 - METF_BUCAP

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei28 – 281Proton donor/acceptorBy similarity
    Binding sitei88 – 881FADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei152 – 1521FADBy similarity
    Binding sitei183 – 1831SubstrateBy similarity
    Binding sitei219 – 2191SubstrateBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Binding sitei275 – 2751SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 336NADBy similarity
    Nucleotide bindingi59 – 624FADBy similarity
    Nucleotide bindingi59 – 602NADBy similarity
    Nucleotide bindingi118 – 1203FADBy similarity
    Nucleotide bindingi131 – 1322FADBy similarity
    Nucleotide bindingi156 – 1594FADBy similarity
    Nucleotide bindingi165 – 1728FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-48-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    Ordered Locus Names:BUsg_043
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2922925,10-methylenetetrahydrofolate reductasePRO_0000190259Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi198804.BUsg043.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8KA62.
    SMRiQ8KA62. Positions 22-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    KOiK00297.
    OMAiHENGSSH.
    OrthoDBiEOG6D2KVW.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8KA62-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNFLHQYYQD IIKKKLENLN DKIQCSFEFF PPKNLDTEKK LFSSVVKLSL    50
    LKPFLFSVTY GANSGERKKT YSIVQRIHKK TGITTAPHLT CVDASLSELK 100
    EIAKFYWENG IRSIVALRGD VPNKSYQHKI YALDLVVLLK KIADFDISVA 150
    AYPEIHPESK NAQSDILNLK KKADAGANRA ITQFFFNVES YLRFRDNCIK 200
    NNINIEIIPG ILPIYNFEQL KKFSSMTNVS IPKWMFEMFN GLDEDLETQK 250
    IIGSNIVINI VKTLLYEGVK NFHFYTLNKS DVIYSICHMF GL 292
    Length:292
    Mass (Da):33,676
    Last modified:October 1, 2002 - v1
    Checksum:i0660895E1B065E71
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67614.1.
    RefSeqiNP_660403.1. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM67614; AAM67614; BUsg_043.
    GeneIDi1005859.
    KEGGibas:BUsg043.
    PATRICi21246851. VBIBucAph100086_0043.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67614.1 .
    RefSeqi NP_660403.1. NC_004061.1.

    3D structure databases

    ProteinModelPortali Q8KA62.
    SMRi Q8KA62. Positions 22-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg043.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM67614 ; AAM67614 ; BUsg_043 .
    GeneIDi 1005859.
    KEGGi bas:BUsg043.
    PATRICi 21246851. VBIBucAph100086_0043.

    Phylogenomic databases

    eggNOGi COG0685.
    KOi K00297.
    OMAi HENGSSH.
    OrthoDBi EOG6D2KVW.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci BAPH198804:GHMG-48-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiMETF_BUCAP
    AccessioniPrimary (citable) accession number: Q8KA62
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3