ID   ASSY_BUCAP              Reviewed;         401 AA.
AC   Q8KA60;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 48.
DE   RecName: Full=Argininosuccinate synthase;
DE            EC=6.3.4.5;
DE   AltName: Full=Citrulline--aspartate ligase;
GN   Name=argG; OrderedLocusNames=BUsg_047;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP +
CC       diphosphate + N(omega)-(L-arginino)succinate.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-
CC       arginine from L-ornithine and carbamoyl phosphate: step 2/3.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type
CC       1 subfamily.
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DR   EMBL; AE013218; AAM67618.1; -; Genomic_DNA.
DR   RefSeq; NP_660407.1; -.
DR   GeneID; 1005863; -.
DR   GenomeReviews; AE013218_GR; BUsg_047.
DR   KEGG; bas:BUsg047; -.
DR   HOGENOM; Q8KA60; -.
DR   OMA; Q8KA60; WAARNGR.
DR   BioCyc; BAPH198804:BUSG047-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00005; -; 1.
DR   InterPro; IPR001518; Arginosuc_synth.
DR   InterPro; IPR018223; Arginosuc_synth_CS.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR11587; Arginosuc_synth; 1.
DR   Pfam; PF00764; Arginosuc_synth; 1.
DR   TIGRFAMs; TIGR00032; argG; 1.
DR   PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1.
DR   PROSITE; PS00565; ARGININOSUCCIN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW   Complete proteome; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN         1    401       Argininosuccinate synthase.
FT                                /FTId=PRO_0000148577.
FT   NP_BIND      10     18       ATP (By similarity).
FT   BINDING      37     37       ATP; via amide nitrogen and carbonyl
FT                                oxygen (By similarity).
FT   BINDING      89     89       Citrulline (By similarity).
FT   BINDING     119    119       ATP; via amide nitrogen (By similarity).
FT   BINDING     121    121       Aspartate (By similarity).
FT   BINDING     125    125       Aspartate (By similarity).
FT   BINDING     125    125       Citrulline (By similarity).
FT   BINDING     126    126       Aspartate (By similarity).
FT   BINDING     129    129       Citrulline (By similarity).
FT   BINDING     178    178       Citrulline (By similarity).
FT   BINDING     187    187       Citrulline (By similarity).
FT   BINDING     263    263       Citrulline (By similarity).
FT   BINDING     275    275       Citrulline (By similarity).
SQ   SEQUENCE   401 AA;  45124 MW;  16DD4B6F98E13D8C CRC64;
     MNNFKKVVLA YSGGLDTSAI IPWLKENYHV DVVAFVADIG QSKKDLNGIE KKALQSGAID
     CRVFDLKEEF IKDYVYPVLK TGSLYEGNYL LGTAIARPII AKKQVEFASS IGAKFLCHGA
     TGKGNDQVRF EIAYAALAPY MKVIAPWREW NLNSRESLLE YLREKNIPTS ATLEKIYSRD
     ENSLHISTEG GLLEDLWNKP NADCWNWTVE LEDAPEQPEY ISLIVKNGSV VSVNNEFLTP
     LKCIEKLNKI GSKHAIGRID IVENRLVGIK SRGCYETPGG TIMHIALRAI EQLVFDRESF
     KWREKIGLEM SSVVYDGRWF TPIRKSLQAS ADSLSSEVNG EVVLKLYKGS VIPVQKKSSN
     SLYSKEYATF GEDEVYKHSD AEGFIRLFSL SSRIRAQNKL K
//