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Reviewed, UniProtKB/Swiss-Prot Q8KA60 (ASSY_BUCAP)

Last modified February 9, 2010. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase
Gene names
Name: argG
Ordered Locus Names: BUsg_047
OrganismBuchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]
Taxonomic identifier98794 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

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Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005

Subunit structure

Homotetramer By similarity. HAMAP MF_00005

Subcellular location

Cytoplasm Probable HAMAP MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

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Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401Argininosuccinate synthase HAMAP MF_00005
PRO_0000148577

Regions

Nucleotide binding10 – 189ATP By similarity

Sites

Binding site371ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site891Citrulline By similarity
Binding site1191ATP; via amide nitrogen By similarity
Binding site1211Aspartate By similarity
Binding site1251Aspartate By similarity
Binding site1251Citrulline By similarity
Binding site1261Aspartate By similarity
Binding site1291Citrulline By similarity
Binding site1781Citrulline By similarity
Binding site1871Citrulline By similarity
Binding site2631Citrulline By similarity
Binding site2751Citrulline By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q8KA60-1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 16DD4B6F98E13D8C

FASTA40145,124
        10         20         30         40         50         60 
MNNFKKVVLA YSGGLDTSAI IPWLKENYHV DVVAFVADIG QSKKDLNGIE KKALQSGAID 

        70         80         90        100        110        120 
CRVFDLKEEF IKDYVYPVLK TGSLYEGNYL LGTAIARPII AKKQVEFASS IGAKFLCHGA 

       130        140        150        160        170        180 
TGKGNDQVRF EIAYAALAPY MKVIAPWREW NLNSRESLLE YLREKNIPTS ATLEKIYSRD 

       190        200        210        220        230        240 
ENSLHISTEG GLLEDLWNKP NADCWNWTVE LEDAPEQPEY ISLIVKNGSV VSVNNEFLTP 

       250        260        270        280        290        300 
LKCIEKLNKI GSKHAIGRID IVENRLVGIK SRGCYETPGG TIMHIALRAI EQLVFDRESF 

       310        320        330        340        350        360 
KWREKIGLEM SSVVYDGRWF TPIRKSLQAS ADSLSSEVNG EVVLKLYKGS VIPVQKKSSN 

       370        380        390        400 
SLYSKEYATF GEDEVYKHSD AEGFIRLFSL SSRIRAQNKL K

« Hide

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References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE013218 Genomic DNA. Translation: AAM67618.1.
RefSeqNP_660407.1.

3D structure databases

SMRQ8KA60. Positions 6-399.
ModBaseSearch...

Genome annotation databases

GeneID1005863.
GenomeReviewsGene locus BUsg_047 in contig AE013218_GR.
KEGGbas:BUsg047.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG335267.
OMAWAARNGR.

Enzyme and pathway databases

BioCycBAPH198804:BUSG047-MONOMER.

Family and domain databases

HAMAPMF_00005. Arg_succ_synth_type1.
[Tree]
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHERPTHR11587. Arginosuc_synth. 1 hit.
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameASSY_BUCAP
AccessionPrimary (citable) accession number: Q8KA60
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents