ID   CCA_BUCAP               Reviewed;         412 AA.
AC   Q8KA53;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   05-MAY-2009, entry version 43.
DE   RecName: Full=CCA-adding enzyme;
DE            EC=2.7.7.25;
DE            EC=2.7.7.21;
DE   AltName: Full=tRNA nucleotidyltransferase;
DE   AltName: Full=tRNA adenylyl-/cytidylyl- transferase;
DE   AltName: Full=tRNA CCA-pyrophosphorylase;
DE   AltName: Full=tRNA-NT;
GN   Name=cca; OrderedLocusNames=BUsg_058;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Catalyzes the addition and repair of the essential 3'-
CC       terminal CCA sequence in tRNAs without using a nucleic acid
CC       template. Adds these three nucleotides in the order of C, C, and A
CC       to the tRNA nucleotide-73, using CTP and ATP as substrates and
CC       producing inorganic pyrophosphate (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + tRNA(n) = diphosphate + tRNA(n+1).
CC   -!- CATALYTIC ACTIVITY: CTP + tRNA(n) = diphosphate + tRNA(n+1).
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- MISCELLANEOUS: A single active site specifically recognizes both
CC       ATP and CTP and is responsible for their addition (By similarity).
CC   -!- SIMILARITY: Belongs to the tRNA nucleotidyltransferase/poly(A)
CC       polymerase family. Bacterial CCA-adding enzyme type 2 subfamily.
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DR   EMBL; AE013218; AAM67629.1; -; Genomic_DNA.
DR   RefSeq; NP_660418.1; -.
DR   GeneID; 1005874; -.
DR   GenomeReviews; AE013218_GR; BUsg_058.
DR   KEGG; bas:BUsg058; -.
DR   HOGENOM; Q8KA53; -.
DR   OMA; Q8KA53; NPHVYFQ.
DR   BioCyc; BAPH198804:BUSG058-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR   GO; GO:0004810; F:tRNA adenylyltransferase activity; IEA:HAMAP.
DR   GO; GO:0000049; F:tRNA binding; IEA:HAMAP.
DR   GO; GO:0016437; F:tRNA cytidylyltransferase activity; IEA:HAMAP.
DR   GO; GO:0042245; P:RNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0001680; P:tRNA 3'-terminal CCA addition; IEA:HAMAP.
DR   HAMAP; MF_01262; -; 1.
DR   InterPro; IPR012006; CCA_bact.
DR   InterPro; IPR002646; PolyA_pol_reg.
DR   Pfam; PF01743; PolyA_pol; 1.
DR   PIRSF; PIRSF000813; CCA_bact; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Magnesium; Metal-binding;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA repair; RNA-binding;
KW   Transferase; tRNA processing.
FT   CHAIN         1    412       CCA-adding enzyme.
FT                                /FTId=PRO_0000139018.
FT   METAL        21     21       Magnesium (By similarity).
FT   METAL        23     23       Magnesium (By similarity).
FT   BINDING       8      8       ATP or CTP; via amide nitrogen (By
FT                                similarity).
FT   BINDING      11     11       ATP or CTP (By similarity).
FT   BINDING      91     91       ATP or CTP (By similarity).
FT   BINDING     137    137       ATP or CTP (By similarity).
FT   BINDING     140    140       ATP or CTP (By similarity).
SQ   SEQUENCE   412 AA;  48583 MW;  B0A850ECD5D20C22 CRC64;
     MKVYLVGGAV RDSLLNLPIK DRDWVIVGGT KEILLKKNFQ QVGKDFPVFL HPETHEEYSL
     ARKERKSGRG YTGFHTEFSS DVTLEEDLIR RDLTINAIAQ DENGNYIDPF QGRKDLNLRL
     LRHVSESFTE DPLRILRTAR FAANLMHLGF HIDKDTMILM CKMVKKNELL YLTKNRIWNE
     TEKAFKTLNP HVYFQILHAC KALNFIFPEI FFLYERRTFF SILFTNFYSI SFLSIGLSKI
     STLTKDVSIR FSYLCQFLSE KIDFSSTKEN YDDDSAKLVK KLCKRCNIPS HIEELAVLNT
     GFCVFLSSIH YQSSSNIIKM FSKIDAWRKP DRIYKLSFLC DFNLFYHQNK IDNSKLKTGF
     LKKCFFIIKD ISVKKILNQG FKGYQIKNEL NKLRVNKLKF WRMKKKCFFF KE
//