Q8KA53 (CCA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCA-adding enzyme
EC=2.7.7.72

Alternative name(s):
CCA tRNA nucleotidyltransferase
tRNA CCA-pyrophosphorylase
tRNA adenylyl-/cytidylyl- transferase
tRNA nucleotidyltransferase
tRNA-NT

Gene names

Name:	cca
Ordered Locus Names:	BUsg_058

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity. HAMAP MF_01262
Catalytic activity	A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate. HAMAP MF_01262
Cofactor	Magnesium By similarity. HAMAP MF_01262
Miscellaneous	A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity. HAMAP MF_01262
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily.

Ontologies

Keywords
Biological process	RNA repair tRNA processing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA repair Inferred from electronic annotation. Source: UniProtKB-KW tRNA 3'-terminal CCA addition Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA adenylyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	CCA-adding enzyme HAMAP MF_01262		PRO_0000139018
Sites
	Metal binding	21	1	Magnesium By similarity
	Metal binding	23	1	Magnesium By similarity
	Binding site	8	1	ATP or CTP; via amide nitrogen By similarity
	Binding site	11	1	ATP or CTP By similarity
	Binding site	91	1	ATP or CTP By similarity
	Binding site	137	1	ATP or CTP By similarity
	Binding site	140	1	ATP or CTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA53 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B0A850ECD5D20C22	FASTA	412	48,583
	10 20 30 40 50 60 MKVYLVGGAV RDSLLNLPIK DRDWVIVGGT KEILLKKNFQ QVGKDFPVFL HPETHEEYSL 70 80 90 100 110 120 ARKERKSGRG YTGFHTEFSS DVTLEEDLIR RDLTINAIAQ DENGNYIDPF QGRKDLNLRL 130 140 150 160 170 180 LRHVSESFTE DPLRILRTAR FAANLMHLGF HIDKDTMILM CKMVKKNELL YLTKNRIWNE 190 200 210 220 230 240 TEKAFKTLNP HVYFQILHAC KALNFIFPEI FFLYERRTFF SILFTNFYSI SFLSIGLSKI 250 260 270 280 290 300 STLTKDVSIR FSYLCQFLSE KIDFSSTKEN YDDDSAKLVK KLCKRCNIPS HIEELAVLNT 310 320 330 340 350 360 GFCVFLSSIH YQSSSNIIKM FSKIDAWRKP DRIYKLSFLC DFNLFYHQNK IDNSKLKTGF 370 380 390 400 410 LKKCFFIIKD ISVKKILNQG FKGYQIKNEL NKLRVNKLKF WRMKKKCFFF KE

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67629.1.
RefSeq	NP_660418.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8KA53.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000291; EBBUCP00000000291; EBBUCG00000000291.
GeneID	1005874.
GenomeReviews	Gene locus BUsg_058 in contig AE013218_GR.
KEGG	bas:BUsg058.
PATRIC	21246881. VBIBucAph100086_0058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008009.
HOGENOM	HBG635063.
OMA	QVGKGFP.
ProtClustDB	PRK13298.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG058-MONOMER.
Family and domain databases
HAMAP	MF_01262. CCA_bact_type2. [Tree]
InterPro	IPR012006. CCA_bact. IPR002646. PolA_pol_head_dom. [Graphical view]
KO	K00974.
Pfam	PF01743. PolyA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000813. CCA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCA_BUCAP

Accession

Primary (citable) accession number: Q8KA53

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program