Reviewed, UniProtKB/Swiss-Prot Q8KA53 (CCA_BUCAP)

Names and origin

Protein names

Recommended name:
    CCA-adding enzyme
    EC=2.7.7.25
    EC=2.7.7.21
Alternative name(s):
    tRNA nucleotidyltransferase
    tRNA adenylyl-/cytidylyl- transferase
    tRNA CCA-pyrophosphorylase
    tRNA-NT

Gene names

Name:	cca
Ordered Locus Names:	BUsg_058

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity.
Catalytic activity	ATP + tRNA(n) = diphosphate + tRNA(n+1). HAMAP MF_01262 CTP + tRNA(n) = diphosphate + tRNA(n+1). HAMAP MF_01262
Cofactor	Magnesium By similarity.
Miscellaneous	A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity.
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily.

Ontologies

Keywords
Biological process	RNA repair tRNA processing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA repair Inferred from electronic annotation. Source: UniProtKB-KW tRNA 3'-terminal CCA addition Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP tRNA adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	CCA-adding enzyme HAMAP MF_01262		PRO_0000139018
Sites
	Metal binding	21	1	Magnesium By similarity
	Metal binding	23	1	Magnesium By similarity
	Binding site	8	1	ATP or CTP; via amide nitrogen By similarity
	Binding site	11	1	ATP or CTP By similarity
	Binding site	91	1	ATP or CTP By similarity
	Binding site	137	1	ATP or CTP By similarity
	Binding site	140	1	ATP or CTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA53-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B0A850ECD5D20C22	FASTA	412	48,583
	10 20 30 40 50 60 MKVYLVGGAV RDSLLNLPIK DRDWVIVGGT KEILLKKNFQ QVGKDFPVFL HPETHEEYSL 70 80 90 100 110 120 ARKERKSGRG YTGFHTEFSS DVTLEEDLIR RDLTINAIAQ DENGNYIDPF QGRKDLNLRL 130 140 150 160 170 180 LRHVSESFTE DPLRILRTAR FAANLMHLGF HIDKDTMILM CKMVKKNELL YLTKNRIWNE 190 200 210 220 230 240 TEKAFKTLNP HVYFQILHAC KALNFIFPEI FFLYERRTFF SILFTNFYSI SFLSIGLSKI 250 260 270 280 290 300 STLTKDVSIR FSYLCQFLSE KIDFSSTKEN YDDDSAKLVK KLCKRCNIPS HIEELAVLNT 310 320 330 340 350 360 GFCVFLSSIH YQSSSNIIKM FSKIDAWRKP DRIYKLSFLC DFNLFYHQNK IDNSKLKTGF 370 380 390 400 410 LKKCFFIIKD ISVKKILNQG FKGYQIKNEL NKLRVNKLKF WRMKKKCFFF KE

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67629.1.
RefSeq	NP_660418.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005874.
GenomeReviews	Gene locus BUsg_058 in contig AE013218_GR.
KEGG	bas:BUsg058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8KA53.
OMA	ADHAGRL
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG058-MON.
Family and domain databases
HAMAP	MF_01262. [Tree]
InterPro	IPR012006. CCA_bact. IPR002646. PolyA_pol_reg. [Graphical view]
Pfam	PF01743. PolyA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000813. CCA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCA_BUCAP

Accession

Primary (citable) accession number: Q8KA53

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	November 24, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)