Q8KA49 (PTHP_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphocarrier protein HPr
EC=2.7.11.-

Alternative name(s):
Histidine-containing protein

Gene names

Name:	ptsH
Ordered Locus Names:	BUsg_062

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III) By similarity.
Catalytic activity	Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HPr family. Contains 1 HPr domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 85	85	Phosphocarrier protein HPr		PRO_0000107845
Regions
	Domain	1 – 85	85	HPr
Sites
	Active site	15	1	Pros-phosphohistidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA49 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6882A1A6206EAC0E	FASTA	85	9,571
	10 20 30 40 50 60 MFQKEIKINA LHGLHTRPAA EFVKEAKNFI SDIHIIYHGK SVNAKSLFKI QTLGLVKDSV 70 80 IILSAEGEDE KKAVEHLSKI MTELE

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67633.1.
RefSeq	NP_660422.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8KA49.
SMR	Q8KA49. Positions 1-85.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg062.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67633; AAM67633; BUsg_062.
GeneID	1005878.
KEGG	bas:BUsg062.
PATRIC	21246889. VBIBucAph100086_0062.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1925.
KO	K02784.
OMA	CSIIVEC.
ProtClustDB	CLSK315127.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-134-MONOMER.
Family and domain databases
Gene3D	3.30.1340.10. 1 hit.
InterPro	IPR001020. PTS_HPr_His_P_site. IPR005698. PTS_HPr_prot. IPR000032. PTS_HPr_prot-like. IPR002114. PTS_HPr_Ser_P_site. [Graphical view]
Pfam	PF00381. PTS-HPr. 1 hit. [Graphical view]
PRINTS	PR00107. PHOSPHOCPHPR.
SUPFAM	SSF55594. HPr_protein. 1 hit.
TIGRFAMs	TIGR01003. PTS_HPr_family. 1 hit.
PROSITE	PS51350. PTS_HPR_DOM. 1 hit. PS00369. PTS_HPR_HIS. 1 hit. PS00589. PTS_HPR_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTHP_BUCAP

Accession

Primary (citable) accession number: Q8KA49

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program