Reviewed, UniProtKB/Swiss-Prot Q8KA48 (CYSK_BUCAP)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	cysK
Ordered Locus Names:	BUsg_063

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Cysteine synthase		PRO_0000167084
Regions
	Region	177 – 181	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	8	1	Allosteric inhibitor By similarity
	Binding site	72	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Allosteric inhibitor; via amide nitrogen By similarity
	Binding site	273	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	42	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA48-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 52BA593760519D52	FASTA	322	35,177
	10 20 30 40 50 60 MNKIYEDNSF TIGNTPLVRL NNIGNGNILA KIESRNPSFS VKCRIGANMI WDAEKKGRLN 70 80 90 100 110 120 KNIEIIEATS GNTGIALAYV AAARNYSLTL IMPDSMSIER RKLLKSLGAH LILTNGKHGM 130 140 150 160 170 180 KGAISKANEI VSLNTKRYLL LKQFENPANP EIHQKTTGPE IWKDTKGNID ILISGVGTGG 190 200 210 220 230 240 TITGITKYIK IKEGKKDLIS VAVEPLESPV ITQFLSGKKI KPGLHKIQGI GAGFIPKNLD 250 260 270 280 290 300 LSLIDRIITV SSEEAIFNAK KIMKKEGILS GISSGAAIAA ALKIQKENDF KNKNIVVILP 310 320 SSGERYLSTE LFSRSFKNEN NY

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67634.1.
RefSeq	NP_660423.1.
3D structure databases
HSSP	HSSP built from PDB template 1OAS based on UniProtKB P12674.
SMR	Q8KA48. Positions 2-316.
ModBase	Search...
Genome annotation databases
GeneID	1005879.
GenomeReviews	Gene locus BUsg_063 in contig AE013218_GR.
KEGG	bas:BUsg063.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8KA48.
OMA	KYFMPGQ.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG063-MON.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_BUCAP

Accession

Primary (citable) accession number: Q8KA48

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)