Reviewed, UniProtKB/Swiss-Prot Q8KA47 (SYE_BUCAP)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase
      Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	BUsg_065

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Glutamyl-tRNA synthetase HAMAP MF_00022		PRO_0000119527
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022
	Motif	238 – 242	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	241	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA47-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 07D3789761DEDFA2	FASTA	474	55,412
	10 20 30 40 50 60 MKVKTRFAPS PTGNLHIGSI RTALYSWLFA RHYHGKFILR IEDTDFERFD SKSVESILYG 70 80 90 100 110 120 LKWLGLNWDE GPYFQSKRLE RYKEVIKMML ESGNAYKCFC SVKELEDERI RQVSKGKKPR 130 140 150 160 170 180 YSGICRNLHY RNYIANENYV VRFKNPISGQ VTFEDKIRGK ITFQNEELDD LVIQRSNGIP 190 200 210 220 230 240 TYNFCVVIDD LDMNITHVIR GEDHINNTPR QINILKSLNA KIPIYAHVSM IFNEDGHKFS 250 260 270 280 290 300 KRQDALNILE YCKDGFLPEA LLNYVIRLGW SCGNQEIFSI SEMKELFDLR KISKSSSAVS 310 320 330 340 350 360 MKKLFWLNKY YINNLPLSYI SNLLKDHMNH EKINLKNGPD LESVLTLLKN RHHTLKEIAQ 370 380 390 400 410 420 SSRYFYEEFA NFDKSAADEY LIPENHFILK NLYKNIKKIS IWSNETLSSL IKNDCIKLKI 430 440 450 460 470 TLRKISMLLR VVLTGNIFSP SISSVIFLIG KEKTLLRLKK AILFIDNKIK NINN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67635.1.
RefSeq	NP_660424.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005881.
GenomeReviews	Gene locus BUsg_065 in contig AE013218_GR.
KEGG	bas:BUsg065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG065-MON.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_BUCAP

Accession

Primary (citable) accession number: Q8KA47

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	December 15, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)