Q8KA31 (IPYR_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	BUsg_081

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137485
Sites
	Metal binding	66	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 2 By similarity
	Metal binding	103	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA31 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B954B88307F091EF	FASTA	180	20,510
	10 20 30 40 50 60 MNLNLIKAGN NIPSDIYVII EISSNSSPIK YEVDKQSGVL FVDRFIPTPM FYPCNYGYIN 70 80 90 100 110 120 ETLSLDGDPL DVLVPSPYPI QSNVVINCKP VGILKMHDES GNDAKIIAVP NDKVSKEYEN 130 140 150 160 170 180 INDISDISEL LKKQISHFFQ YYKTLEKEKW VEIIGWGDHH EAKLEIKNSY DRAKKNTSLK

References

[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67651.1.
RefSeq	NP_660440.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8KA31.
SMR	Q8KA31. Positions 6-174.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000399; EBBUCP00000000399; EBBUCG00000000399.
GeneID	1005898.
GenomeReviews	Gene locus BUsg_081 in contig AE013218_GR.
KEGG	bas:BUsg081.
PATRIC	21246935. VBIBucAph100086_0082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007981.
HOGENOM	HBG529150.
OMA	AMVIMEE.
ProtClustDB	PRK01250.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG081-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_BUCAP

Accession

Primary (citable) accession number: Q8KA31

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program