Reviewed, UniProtKB/Swiss-Prot Q8KA24 (DAPA_BUCAP)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	BUsg_088

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000103091
Regions
	Region	48 – 49	2	Pyruvate binding By similarity
Sites
	Active site	161	1	Schiff-base intermediate with substrate By similarity
	Binding site	106	1	Pyruvate By similarity
	Site	133	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8KA24-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: DFA9469F851DC221	FASTA	294	32,452
	10 20 30 40 50 60 MFKGSIVALI TPMNEEGEIC HSSLKKLIDY HVLNKTKAIV SIGTTGESAT LSQEEHIEVV 70 80 90 100 110 120 MLTLKLANKR IPIIAGTGAN ATTEAISLTK RFEKSGIEAC LTVTPYYNKP TQEGLYQHFK 130 140 150 160 170 180 AISENTKLPQ ILYNVPSRTG CDLLPSTVAR LSEFKNIIGI KEATGDLSRI HKIKKLVKDD 190 200 210 220 230 240 FLLISGDDPT ALDFIQLGGQ GVISVTANIA AKEMTQMCSY ALEGNFKSAR SINERLMLLH 250 260 270 280 290 ESLFIEPNPI PIKWLAKKIG LIKSDTLRLP MTPILNSTRT QIEKALQYAN LQKI

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67658.1.
RefSeq	NP_660447.1.
3D structure databases
SMR	Q8KA24. Positions 1-291.
ModBase	Search...
Genome annotation databases
GeneID	1005905.
GenomeReviews	Gene locus BUsg_088 in contig AE013218_GR.
KEGG	bas:BUsg088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG358848.
OMA	HQKLFVE.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG088-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. Dihydrodipicolinate_synth. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_BUCAP

Accession

Primary (citable) accession number: Q8KA24

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)