Q8KA22 (RISA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase alpha chain
EC=2.5.1.9

Gene names

Name:	ribE
Ordered Locus Names:	BUsg_104

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Riboflavin synthase alpha chain		PRO_0000068161
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 195	98	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	Q8KA22 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: CE00EE91A2C732AF	FASTA	208	23,365
	10 20 30 40 50 60 MFTGIVNGIA RVVSINKKKN FHTYTVNFSS ILLKNLKIGD SVAHNGCCLT VKYINCPHVV 70 80 90 100 110 120 FDIMKITIAN TNLGVLNIGD YVNIERSLKY GDEMGGHIIS GHIMNTGEIS KISKLDKNYI 130 140 150 160 170 180 LWCKVKDLSL MKYIFYKGFI AIDGISLTIN NIIKNEFCVS IIPETLSSTT IGFKKIGQLV 190 200 NIEIDFYTQI IVDTTKRLIK KDISTLFK

References

[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67674.1.
RefSeq	NP_660463.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8KA22.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000283; EBBUCP00000000283; EBBUCG00000000283.
GeneID	1005921.
GenomeReviews	Gene locus BUsg_104 in contig AE013218_GR.
KEGG	bas:BUsg104.
PATRIC	21246987. VBIBucAph100086_0107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007992.
HOGENOM	HBG289809.
OMA	HILSGHV.
ProtClustDB	PRK09289.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG104-MONOMER.
Family and domain databases
InterPro	IPR023366. ATPase_F1/A1-cplx_a_su_N. IPR001783. Lumazine-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:2.40.30.20. G3DSA:2.40.30.20. 2 hits.
KO	K00793.
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 2 hits.
TIGRFAMs	TIGR00187. RibE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_BUCAP

Accession

Primary (citable) accession number: Q8KA22

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program