Reviewed, UniProtKB/Swiss-Prot Q8KA22 (RISA_BUCAP)

Names and origin

Protein names

Recommended name:
    Riboflavin synthase alpha chain
    EC=2.5.1.9

Gene names

Name:	ribE
Ordered Locus Names:	BUsg_104

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Riboflavin synthase alpha chain		PRO_0000068161
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 195	98	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	Q8KA22-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: CE00EE91A2C732AF	FASTA	208	23,365
	10 20 30 40 50 60 MFTGIVNGIA RVVSINKKKN FHTYTVNFSS ILLKNLKIGD SVAHNGCCLT VKYINCPHVV 70 80 90 100 110 120 FDIMKITIAN TNLGVLNIGD YVNIERSLKY GDEMGGHIIS GHIMNTGEIS KISKLDKNYI 130 140 150 160 170 180 LWCKVKDLSL MKYIFYKGFI AIDGISLTIN NIIKNEFCVS IIPETLSSTT IGFKKIGQLV 190 200 NIEIDFYTQI IVDTTKRLIK KDISTLFK

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67674.1.
RefSeq	NP_660463.1.
3D structure databases
SMR	Q8KA22. Positions 1-194.
ModBase	Search...
Genome annotation databases
GeneID	1005921.
GenomeReviews	Gene locus BUsg_104 in contig AE013218_GR.
KEGG	bas:BUsg104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289809.
OMA	DEIGGHS.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG104-MONOMER.
Family and domain databases
InterPro	IPR001783. Lumazine_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_BUCAP

Accession

Primary (citable) accession number: Q8KA22

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)