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Q8KA16 (END3_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endonuclease III

EC=4.2.99.18
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene names
Name:nth
Ordered Locus Names:BUsg_111
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity. HAMAP-Rule MF_00942

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00942

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_00942

Sequence similarities

Belongs to the Nth/MutY family.

Contains 1 HhH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Endonuclease III HAMAP-Rule MF_00942
PRO_0000102215

Regions

Domain108 – 12720HhH

Sites

Metal binding1871Iron-sulfur (4Fe-4S) By similarity
Metal binding1941Iron-sulfur (4Fe-4S) By similarity
Metal binding1971Iron-sulfur (4Fe-4S) By similarity
Metal binding2031Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8KA16 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 8C2E141DA3FD1FCB

FASTA20924,074
        10         20         30         40         50         60 
MNKKKRFEIL SLFYKKNSNP KIELVFSSDF ELLLSVILSA KSTDVMVNKI TGTLFQIANT 

        70         80         90        100        110        120 
PQSILKLGFN KLRHYIKSIG LYNTKSLNII NSAYLIKTKY NNKVPSNRTE LESLPGVGRK 

       130        140        150        160        170        180 
TANIILNVLF NKNTIAVDTH VFRVANRTGF AKGKNVIEVE KKMIKIVPSI FKKYVHFWFV 

       190        200 
LHGRYVCTAR QLKCKTCFIE KLCEFDKKK 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67680.1.
RefSeqNP_660469.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8KA16.
SMRQ8KA16. Positions 1-208.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg111.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67680; AAM67680; BUsg_111.
GeneID1005928.
KEGGbas:BUsg111.
PATRIC21247003. VBIBucAph100086_0115.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0177.
KOK10773.
OMADTHIYRV.
OrthoDBEOG6H4KC5.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-121-MONOMER.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPMF_00942. Nth.
InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFPIRSF001435. Nth. 1 hit.
SMARTSM00478. ENDO3c. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. SSF48150. 1 hit.
TIGRFAMsTIGR01083. nth. 1 hit.
PROSITEPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND3_BUCAP
AccessionPrimary (citable) accession number: Q8KA16
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names