Reviewed, UniProtKB/Swiss-Prot Q8K9Z8 (DYR_BUCAP)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
    EC=1.5.1.3

Gene names

Name:	folA
Ordered Locus Names:	BUsg_136

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Dihydrofolate reductase		PRO_0000186384
Regions
	Domain	2 – 161	160	DHFR
	Nucleotide binding	7 – 8	2	NADP By similarity
	Nucleotide binding	15 – 20	6	NADP By similarity
	Nucleotide binding	44 – 47	4	NADP By similarity
	Nucleotide binding	64 – 66	3	NADP By similarity
	Nucleotide binding	96 – 101	6	NADP By similarity
	Region	6 – 8	3	Substrate binding By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	59	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9Z8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1E86675D87DC1B4D	FASTA	161	19,113
	10 20 30 40 50 60 MKISLISAIS NNLVIGHNNK IPWYLPEDLK WFKKNTIHKD IIMGRLTWES ILNHPLPMRK 70 80 90 100 110 120 NIVISHKEII HKDVIWANSI SKALLSTTYD KEIMVIGGSK IYKQMLFYAT KLYLTHVDFN 130 140 150 160 GIGDTYFPQY KSCKFWKTIF RKQHFKDKNN PYNFCFEILS R

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67704.1.
RefSeq	NP_660493.1.
3D structure databases
SMR	Q8K9Z8. Positions 1-161.
ModBase	Search...
Genome annotation databases
GeneID	1005953.
GenomeReviews	Gene locus BUsg_136 in contig AE013218_GR.
KEGG	bas:BUsg136.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG665708.
OMA	TIWHEKS.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG136-MONOMER.
Family and domain databases
InterPro	IPR012259. DHFR. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_BUCAP

Accession

Primary (citable) accession number: Q8K9Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)