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Q8K9Z8 (DYR_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:folA
Ordered Locus Names:BUsg_136
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Dihydrofolate reductase
PRO_0000186384

Regions

Domain2 – 161160DHFR
Nucleotide binding7 – 82NADP By similarity
Nucleotide binding15 – 206NADP By similarity
Nucleotide binding44 – 474NADP By similarity
Nucleotide binding64 – 663NADP By similarity
Nucleotide binding96 – 1016NADP By similarity
Region6 – 83Substrate binding By similarity

Sites

Binding site281Substrate By similarity
Binding site591Substrate By similarity
Binding site1151Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9Z8 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 1E86675D87DC1B4D

FASTA16119,113
        10         20         30         40         50         60 
MKISLISAIS NNLVIGHNNK IPWYLPEDLK WFKKNTIHKD IIMGRLTWES ILNHPLPMRK 

        70         80         90        100        110        120 
NIVISHKEII HKDVIWANSI SKALLSTTYD KEIMVIGGSK IYKQMLFYAT KLYLTHVDFN 

       130        140        150        160 
GIGDTYFPQY KSCKFWKTIF RKQHFKDKNN PYNFCFEILS R 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67704.1.
RefSeqNP_660493.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9Z8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg136.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67704; AAM67704; BUsg_136.
GeneID1005953.
KEGGbas:BUsg136.
PATRIC21247053. VBIBucAph100086_0140.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0262.
KOK00287.
OMADTQFPDW.
OrthoDBEOG6KT2V2.
ProtClustDBCLSK315509.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-146-MONOMER.
UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_BUCAP
AccessionPrimary (citable) accession number: Q8K9Z8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: November 13, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names