ID   CARA_BUCAP              Reviewed;         385 AA.
AC   Q8K9Z6;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Carbamoyl-phosphate synthase small chain;
DE            EC=6.3.5.5;
DE   AltName: Full=Carbamoyl-phosphate synthetase glutamine chain;
GN   Name=carA; OrderedLocusNames=BUsg_138;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- CATALYTIC ACTIVITY: 2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2
CC       ADP + phosphate + L-glutamate + carbamoyl phosphate.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis;
CC       carbamoyl phosphate from HCO(3)(-): step 1/1.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 1/6.
CC   -!- SUBUNIT: Composed of two chains; the small (or glutamine) chain
CC       promotes the hydrolysis of glutamine to ammonia, which is used by
CC       the large (or ammonia) chain to synthesize carbamoyl phosphate (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the carA family.
CC   -!- SIMILARITY: Contains 1 glutamine amidotransferase type-1 domain.
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DR   EMBL; AE013218; AAM67706.1; -; Genomic_DNA.
DR   RefSeq; NP_660495.1; -.
DR   HSSP; P00907; 1CE8.
DR   GeneID; 1005955; -.
DR   GenomeReviews; AE013218_GR; BUsg_138.
DR   KEGG; bas:BUsg138; -.
DR   HOGENOM; Q8K9Z6; -.
DR   OMA; Q8K9Z6; LFDGSNC.
DR   BioCyc; BAPH198804:BUSG138-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004088; F:carbamoyl-phosphate synthase (glutamine-hyd...; IEA:HAMAP.
DR   GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_01209; -; 1.
DR   InterPro; IPR006220; Anth_synthII.
DR   InterPro; IPR001317; CarbamoylP_synth_GATase.
DR   InterPro; IPR006274; CarbamoylP_synth_ssu.
DR   InterPro; IPR002474; CarbamoylP_synth_ssu_N.
DR   InterPro; IPR011702; GATASE.
DR   InterPro; IPR017926; GATASE_1.
DR   InterPro; IPR000991; GATase_class1_C.
DR   PANTHER; PTHR11405:SF4; CarA_synth_small; 1.
DR   Pfam; PF00988; CPSase_sm_chain; 1.
DR   Pfam; PF00117; GATase; 1.
DR   PRINTS; PR00097; ANTSNTHASEII.
DR   PRINTS; PR00099; CPSGATASE.
DR   PRINTS; PR00096; GATASE.
DR   TIGRFAMs; TIGR01368; CPSaseIIsmall; 1.
DR   PROSITE; PS51273; GATASE_TYPE_1; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW   Complete proteome; Glutamine amidotransferase; Ligase;
KW   Nucleotide-binding; Pyrimidine biosynthesis.
FT   CHAIN         1    385       Carbamoyl-phosphate synthase small chain.
FT                                /FTId=PRO_0000112262.
FT   DOMAIN      197    384       Glutamine amidotransferase type-1.
FT   REGION        1    193       CPSase.
FT   ACT_SITE    273    273       Nucleophile (By similarity).
FT   ACT_SITE    357    357       By similarity.
FT   ACT_SITE    359    359       By similarity.
SQ   SEQUENCE   385 AA;  42790 MW;  496864F8283BDE54 CRC64;
     MEDALGQLAV LVLEDGTRFH GRSIGAKGTT VGEVVFNTSI TGYQEIITDP SYSHQIVTLT
     HPHIGNIGTN CNDEESSKIH IRGLIIRDMS PISSNYRSEK SFSSYLKENN IVAISDIDTR
     KLTRILRTKG SQNGCIIEDK KENYSVAYQK AKKFLSLQGL DLAKKVSTKF IYNWDKGSFF
     INKSKSKLEK KKKFLFHIVV YDFGVKRNIL RMLVDRGCYL TVVPAKTDPK IALNLNPDGI
     FLSNGPGDPR PCDYAIHAIQ CFLKKNIPIF GICLGHQLLA LASGANIIKM KFGHHGGNHP
     VKEIKTNRVI ITSQNHSFTV DAKNMPNNIA ITHSSLFDGT LQGLCLTDKL AFSFQGHPEA
     SPGPHDASSL FDHFIKLLNQ VKFSN
//