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Q8K9Z6 (CARA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Carbamoyl-phosphate synthase small chain

EC=6.3.5.5
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene names
Name:carA
Ordered Locus Names:BUsg_138
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.

Sequence similarities

Belongs to the CarA family.

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385Carbamoyl-phosphate synthase small chain HAMAP MF_01209
PRO_0000112262

Regions

Domain197 – 384188Glutamine amidotransferase type-1
Region1 – 193193CPSase HAMAP MF_01209

Sites

Active site2731Nucleophile By similarity
Active site3571 By similarity
Active site3591 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9Z6 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 496864F8283BDE54

FASTA38542,790
        10         20         30         40         50         60 
MEDALGQLAV LVLEDGTRFH GRSIGAKGTT VGEVVFNTSI TGYQEIITDP SYSHQIVTLT 

        70         80         90        100        110        120 
HPHIGNIGTN CNDEESSKIH IRGLIIRDMS PISSNYRSEK SFSSYLKENN IVAISDIDTR 

       130        140        150        160        170        180 
KLTRILRTKG SQNGCIIEDK KENYSVAYQK AKKFLSLQGL DLAKKVSTKF IYNWDKGSFF 

       190        200        210        220        230        240 
INKSKSKLEK KKKFLFHIVV YDFGVKRNIL RMLVDRGCYL TVVPAKTDPK IALNLNPDGI 

       250        260        270        280        290        300 
FLSNGPGDPR PCDYAIHAIQ CFLKKNIPIF GICLGHQLLA LASGANIIKM KFGHHGGNHP 

       310        320        330        340        350        360 
VKEIKTNRVI ITSQNHSFTV DAKNMPNNIA ITHSSLFDGT LQGLCLTDKL AFSFQGHPEA 

       370        380 
SPGPHDASSL FDHFIKLLNQ VKFSN 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67706.1.
RefSeqNP_660495.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9Z6.
SMRQ8K9Z6. Positions 9-382.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBUCT00000000152; EBBUCP00000000152; EBBUCG00000000152.
GeneID1005955.
GenomeReviewsGene locus BUsg_138 in contig AE013218_GR.
KEGGbas:BUsg138.
PATRIC21247057. VBIBucAph100086_0142.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000008117.
HOGENOMHBG286341.
OMAFTYPELG.
ProtClustDBPRK12564.

Enzyme and pathway databases

BioCycBAPH198804:BUSG138-MONOMER.

Family and domain databases

HAMAPMF_01209. CPSase_S_chain.
[Tree]
InterProIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR017926. GATASE_1.
[Graphical view]
Gene3DG3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KOK01956.
PANTHERPTHR11405:SF4. CarA_synth_small. 1 hit.
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMSSF52021. CP_synthsmall. 1 hit.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCARA_BUCAP
AccessionPrimary (citable) accession number: Q8K9Z6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families