Reviewed, UniProtKB/Swiss-Prot Q8K9Z6 (CARA_BUCAP)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	BUsg_138

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112262
Regions
	Domain	197 – 384	188	Glutamine amidotransferase type-1
	Region	1 – 193	193	CPSase HAMAP MF_01209
Sites
	Active site	273	1	Nucleophile By similarity
	Active site	357	1	By similarity
	Active site	359	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9Z6-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 496864F8283BDE54	FASTA	385	42,790
	10 20 30 40 50 60 MEDALGQLAV LVLEDGTRFH GRSIGAKGTT VGEVVFNTSI TGYQEIITDP SYSHQIVTLT 70 80 90 100 110 120 HPHIGNIGTN CNDEESSKIH IRGLIIRDMS PISSNYRSEK SFSSYLKENN IVAISDIDTR 130 140 150 160 170 180 KLTRILRTKG SQNGCIIEDK KENYSVAYQK AKKFLSLQGL DLAKKVSTKF IYNWDKGSFF 190 200 210 220 230 240 INKSKSKLEK KKKFLFHIVV YDFGVKRNIL RMLVDRGCYL TVVPAKTDPK IALNLNPDGI 250 260 270 280 290 300 FLSNGPGDPR PCDYAIHAIQ CFLKKNIPIF GICLGHQLLA LASGANIIKM KFGHHGGNHP 310 320 330 340 350 360 VKEIKTNRVI ITSQNHSFTV DAKNMPNNIA ITHSSLFDGT LQGLCLTDKL AFSFQGHPEA 370 380 SPGPHDASSL FDHFIKLLNQ VKFSN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67706.1.
RefSeq	NP_660495.1.
3D structure databases
HSSP	HSSP built from PDB template 1CE8 based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1005955.
GenomeReviews	Gene locus BUsg_138 in contig AE013218_GR.
KEGG	bas:BUsg138.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9Z6.
OMA	Q8K9Z6. LFDGSNC.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG138-MON.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_BUCAP

Accession

Primary (citable) accession number: Q8K9Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)