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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotation

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391NADPUniRule annotation
Active sitei159 – 1591Proton donor/acceptorUniRule annotation
Binding sitei160 – 1601SubstrateUniRule annotation
Active sitei163 – 1631Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176NAD(P)UniRule annotation
Nucleotide bindingi102 – 1043NAD(P)UniRule annotation
Nucleotide bindingi126 – 1294NAD(P)UniRule annotation

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate reductase Source: UniProtKB-EC
  2. NAD binding Source: UniProtKB-HAMAP
  3. NADPH binding Source: InterPro
  4. oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-149-MONOMER.
UniPathwayiUPA00034; UER00018.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotation (EC:1.17.1.8UniRule annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:BUsg_139
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2732734-hydroxy-tetrahydrodipicolinate reductasePRO_0000141420Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg139.

Structurei

3D structure databases

ProteinModelPortaliQ8K9Z5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 1702Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the DapB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0289.
KOiK00215.
OMAiCAVYARE.
OrthoDBiEOG6SV5DS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR011770. Dihydrodipicolinate_Rdtase.
IPR023940. Dihydrodipicolinate_Rdtase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9Z5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKKITRIAI TGAMGRMGQV LIKEIQKNKN TVLTAALVKN NHPLIGQNIG
60 70 80 90 100
EKIGIGKTSV SISSDINIEK NDFDVLIDFT KPSGTFYFLE QCYEFKKNMI
110 120 130 140 150
IGTTGFSEKE IKTINSYAKK IALIKASNFS IGINLLYQLI QKTTKILGNT
160 170 180 190 200
SDIDIIEYHH RNKIDIPSGT ALSIGENISK VMNWELNKHS LYYTKGITKK
210 220 230 240 250
IRETKKIGFS SIRSGNIIGK HTVLFSSSDE EIKITHSAFN RESFAKGAIE
260 270
AAVWIHEKKH GLFNMNDILK DKF
Length:273
Mass (Da):30,674
Last modified:October 1, 2002 - v1
Checksum:iE49AD0EADB67042F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67707.1.
RefSeqiNP_660496.1. NC_004061.1.
WP_011053674.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67707; AAM67707; BUsg_139.
GeneIDi1005956.
KEGGibas:BUsg139.
PATRICi21247059. VBIBucAph100086_0143.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67707.1.
RefSeqiNP_660496.1. NC_004061.1.
WP_011053674.1. NC_004061.1.

3D structure databases

ProteinModelPortaliQ8K9Z5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM67707; AAM67707; BUsg_139.
GeneIDi1005956.
KEGGibas:BUsg139.
PATRICi21247059. VBIBucAph100086_0143.

Phylogenomic databases

eggNOGiCOG0289.
KOiK00215.
OMAiCAVYARE.
OrthoDBiEOG6SV5DS.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018.
BioCyciBAPH198804:GHMG-149-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR011770. Dihydrodipicolinate_Rdtase.
IPR023940. Dihydrodipicolinate_Rdtase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiDAPB_BUCAP
AccessioniPrimary (citable) accession number: Q8K9Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.