Q8K9Z3 (LSPA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BUsg_141

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP-Rule MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP-Rule MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP-Rule MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Lipoprotein signal peptidase HAMAP-Rule MF_00161		PRO_0000178772
Regions
	Transmembrane	5 – 27	23	Helical; Potential
	Transmembrane	39 – 58	20	Helical; Potential
	Transmembrane	68 – 85	18	Helical; Potential
	Transmembrane	97 – 119	23	Helical; Potential
	Transmembrane	129 – 149	21	Helical; Potential
Sites
	Active site	111	1	By similarity
	Active site	138	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9Z3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: E5A3D4682F286DC2	FASTA	156	18,748
	10 20 30 40 50 60 MKRKYYWIYI NIIFFIITVD FYSKKWILNH LNIYEKQKVF FILNLFHVHN FGAAFSILSD 70 80 90 100 110 120 QNGWQKYFLL IFSIIIILAI IKIMIKFKKK DKNKILSYSL ILAGAIGNLI DRINYGFVID 130 140 150 FIDLHFKSWH FATFNIADFS IFIGMIMIIK KNYYNS

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67709.1.
RefSeq	NP_660498.1. NC_004061.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg141.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67709; AAM67709; BUsg_141.
GeneID	1005958.
KEGG	bas:BUsg141.
PATRIC	21247063. VBIBucAph100086_0145.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0597.
KO	K03101.
OMA	WLWLAIL.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-211-MONOMER.
UniPathway	UPA00665.
Family and domain databases
HAMAP	MF_00161. LspA.
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BUCAP

Accession

Primary (citable) accession number: Q8K9Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program