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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei563Aminoacyl-adenylateUniRule annotation1
Binding sitei607ATPUniRule annotation1
Metal bindingi903ZincUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi921ZincUniRule annotation1
Metal bindingi924ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-154-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BUsg_142
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983671 – 938Isoleucine--tRNA ligaseAdd BLAST938

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg142

Structurei

3D structure databases

ProteinModelPortaliQ8K9Z2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi604 – 608"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K9Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDYKNTLNL PKTQFSMRAN LSQKEPKILK NWYDNNLYKL IRQKKEGKKI
60 70 80 90 100
FFLHDGPPYA NGNIHIGHAV NKILKDIIIK SKNMSGFDAP YIPSWDCHGL
110 120 130 140 150
PIEQKVEEQM GLKSNNIITT IFQKKCRKYA EKQIKKQKKD FIRLGVIGDW
160 170 180 190 200
ENAHLTMDFK NEANIIKTLS KIIEKKYLYK ESKPIHWCLK CYSSLSDAEI
210 220 230 240 250
EHFDKESDSI FVAIKSKNNK ILKEVLNLNI SSEKDIYLPI WTTTPWTLPS
260 270 280 290 300
SQAITVNPIF EYQIIETKKY NLILAKELVK NVMKILEINH WNILSSFKGK
310 320 330 340 350
ILEGKKFLHP FLENISLPVI LGDHVNLDSG TGAVHTAPDH GPDDYIVSQK
360 370 380 390 400
YKIKTMNLVD FKGNYINNIH PKLNGVNIFQ ANEIIINLLI KKDCLLHHNI
410 420 430 440 450
LKHSYPHCWR HKTPVIFRAT EQWFINIDQK KLRYKTLEEI KKVSWIPKWG
460 470 480 490 500
GSRIEEMIKK RPDWCVSRQR KWGVPMCLFL HKKTGKIHPE NTLLIKKIIK
510 520 530 540 550
KVELEGIEAW WKINLKEMLG ETYNMYNQTF DILDVWFESG NTHTVIKYKN
560 570 580 590 600
KKYSKNHADM FLEGSDQHRG WFMSSLIISM LVKNQSPYSE VLTHGFVVDK
610 620 630 640 650
NGQKMSKSIG NTISPNEVVK TLGGDILRLW VASSNYSNDI SISNEILKRS
660 670 680 690 700
SDIYRRIRNT ARFMLANIND FNPQKNTVLK ENMVLLDRWA ISQAKIVQEE
710 720 730 740 750
IIEFYKKYNF HAIIKRLMYF CSIEMGSFYL DIIKDRQYTL KTNSQERRSC
760 770 780 790 800
QTAIYYIINA LVRWIAPILS FTADEIWNHL PGKHAQYVFT EEWFNKLFDL
810 820 830 840 850
DKNDLFNREF WKNLIEMKNE INKFLETEIK NKNINNSLEA CLILYVTPEV
860 870 880 890 900
KKTLNILGEE LKFIFLTSKV KIELYNTAPI NSTKSKKISN FKIFLKKIKE
910 920 930
KKCPRCWHYN IFIENNNDKI CTRCILNTKG NGEKRFFI
Length:938
Mass (Da):110,233
Last modified:October 1, 2002 - v1
Checksum:iF062A0A37E7132B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA Translation: AAM67710.1
RefSeqiWP_011053677.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM67710; AAM67710; BUsg_142
KEGGibas:BUsg_142

Similar proteinsi

Entry informationi

Entry nameiSYI_BUCAP
AccessioniPrimary (citable) accession number: Q8K9Z2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health