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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
Binding sitei607 – 6071ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-152-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BUsg_142
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Isoleucine--tRNA ligasePRO_0000098367Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg142.

Structurei

3D structure databases

ProteinModelPortaliQ8K9Z2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi604 – 6085"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDYKNTLNL PKTQFSMRAN LSQKEPKILK NWYDNNLYKL IRQKKEGKKI
60 70 80 90 100
FFLHDGPPYA NGNIHIGHAV NKILKDIIIK SKNMSGFDAP YIPSWDCHGL
110 120 130 140 150
PIEQKVEEQM GLKSNNIITT IFQKKCRKYA EKQIKKQKKD FIRLGVIGDW
160 170 180 190 200
ENAHLTMDFK NEANIIKTLS KIIEKKYLYK ESKPIHWCLK CYSSLSDAEI
210 220 230 240 250
EHFDKESDSI FVAIKSKNNK ILKEVLNLNI SSEKDIYLPI WTTTPWTLPS
260 270 280 290 300
SQAITVNPIF EYQIIETKKY NLILAKELVK NVMKILEINH WNILSSFKGK
310 320 330 340 350
ILEGKKFLHP FLENISLPVI LGDHVNLDSG TGAVHTAPDH GPDDYIVSQK
360 370 380 390 400
YKIKTMNLVD FKGNYINNIH PKLNGVNIFQ ANEIIINLLI KKDCLLHHNI
410 420 430 440 450
LKHSYPHCWR HKTPVIFRAT EQWFINIDQK KLRYKTLEEI KKVSWIPKWG
460 470 480 490 500
GSRIEEMIKK RPDWCVSRQR KWGVPMCLFL HKKTGKIHPE NTLLIKKIIK
510 520 530 540 550
KVELEGIEAW WKINLKEMLG ETYNMYNQTF DILDVWFESG NTHTVIKYKN
560 570 580 590 600
KKYSKNHADM FLEGSDQHRG WFMSSLIISM LVKNQSPYSE VLTHGFVVDK
610 620 630 640 650
NGQKMSKSIG NTISPNEVVK TLGGDILRLW VASSNYSNDI SISNEILKRS
660 670 680 690 700
SDIYRRIRNT ARFMLANIND FNPQKNTVLK ENMVLLDRWA ISQAKIVQEE
710 720 730 740 750
IIEFYKKYNF HAIIKRLMYF CSIEMGSFYL DIIKDRQYTL KTNSQERRSC
760 770 780 790 800
QTAIYYIINA LVRWIAPILS FTADEIWNHL PGKHAQYVFT EEWFNKLFDL
810 820 830 840 850
DKNDLFNREF WKNLIEMKNE INKFLETEIK NKNINNSLEA CLILYVTPEV
860 870 880 890 900
KKTLNILGEE LKFIFLTSKV KIELYNTAPI NSTKSKKISN FKIFLKKIKE
910 920 930
KKCPRCWHYN IFIENNNDKI CTRCILNTKG NGEKRFFI
Length:938
Mass (Da):110,233
Last modified:October 1, 2002 - v1
Checksum:iF062A0A37E7132B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67710.1.
RefSeqiWP_011053677.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67710; AAM67710; BUsg_142.
KEGGibas:BUsg142.
PATRICi21247065. VBIBucAph100086_0146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67710.1.
RefSeqiWP_011053677.1. NC_004061.1.

3D structure databases

ProteinModelPortaliQ8K9Z2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM67710; AAM67710; BUsg_142.
KEGGibas:BUsg142.
PATRICi21247065. VBIBucAph100086_0146.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-152-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiSYI_BUCAP
AccessioniPrimary (citable) accession number: Q8K9Z2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: July 22, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.