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Q8K9Z2

- SYI_BUCAP

UniProt

Q8K9Z2 - SYI_BUCAP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
    Binding sitei607 – 6071ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-152-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BUsg_142
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_0000098367Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi198804.BUsg142.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K9Z2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi604 – 6085"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K9Z2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDYKNTLNL PKTQFSMRAN LSQKEPKILK NWYDNNLYKL IRQKKEGKKI    50
    FFLHDGPPYA NGNIHIGHAV NKILKDIIIK SKNMSGFDAP YIPSWDCHGL 100
    PIEQKVEEQM GLKSNNIITT IFQKKCRKYA EKQIKKQKKD FIRLGVIGDW 150
    ENAHLTMDFK NEANIIKTLS KIIEKKYLYK ESKPIHWCLK CYSSLSDAEI 200
    EHFDKESDSI FVAIKSKNNK ILKEVLNLNI SSEKDIYLPI WTTTPWTLPS 250
    SQAITVNPIF EYQIIETKKY NLILAKELVK NVMKILEINH WNILSSFKGK 300
    ILEGKKFLHP FLENISLPVI LGDHVNLDSG TGAVHTAPDH GPDDYIVSQK 350
    YKIKTMNLVD FKGNYINNIH PKLNGVNIFQ ANEIIINLLI KKDCLLHHNI 400
    LKHSYPHCWR HKTPVIFRAT EQWFINIDQK KLRYKTLEEI KKVSWIPKWG 450
    GSRIEEMIKK RPDWCVSRQR KWGVPMCLFL HKKTGKIHPE NTLLIKKIIK 500
    KVELEGIEAW WKINLKEMLG ETYNMYNQTF DILDVWFESG NTHTVIKYKN 550
    KKYSKNHADM FLEGSDQHRG WFMSSLIISM LVKNQSPYSE VLTHGFVVDK 600
    NGQKMSKSIG NTISPNEVVK TLGGDILRLW VASSNYSNDI SISNEILKRS 650
    SDIYRRIRNT ARFMLANIND FNPQKNTVLK ENMVLLDRWA ISQAKIVQEE 700
    IIEFYKKYNF HAIIKRLMYF CSIEMGSFYL DIIKDRQYTL KTNSQERRSC 750
    QTAIYYIINA LVRWIAPILS FTADEIWNHL PGKHAQYVFT EEWFNKLFDL 800
    DKNDLFNREF WKNLIEMKNE INKFLETEIK NKNINNSLEA CLILYVTPEV 850
    KKTLNILGEE LKFIFLTSKV KIELYNTAPI NSTKSKKISN FKIFLKKIKE 900
    KKCPRCWHYN IFIENNNDKI CTRCILNTKG NGEKRFFI 938
    Length:938
    Mass (Da):110,233
    Last modified:October 1, 2002 - v1
    Checksum:iF062A0A37E7132B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67710.1.
    RefSeqiNP_660499.1. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM67710; AAM67710; BUsg_142.
    GeneIDi1005959.
    KEGGibas:BUsg142.
    PATRICi21247065. VBIBucAph100086_0146.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67710.1 .
    RefSeqi NP_660499.1. NC_004061.1.

    3D structure databases

    ProteinModelPortali Q8K9Z2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg142.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM67710 ; AAM67710 ; BUsg_142 .
    GeneIDi 1005959.
    KEGGi bas:BUsg142.
    PATRICi 21247065. VBIBucAph100086_0146.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BAPH198804:GHMG-152-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiSYI_BUCAP
    AccessioniPrimary (citable) accession number: Q8K9Z2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3