Reviewed, UniProtKB/Swiss-Prot Q8K9Z1 (RIBF_BUCAP)

Names and origin

Protein names

Recommended name:
    Riboflavin biosynthesis protein ribF
Including the following 2 domains:
    1- Recommended name:
            Riboflavin kinase
              EC=2.7.1.26
        Alternative name(s):
            Flavokinase
    2- Recommended name:
            FMN adenylyltransferase
              EC=2.7.7.2
        Alternative name(s):
            FAD pyrophosphorylase
            FAD synthetase

Gene names

Name:	ribF
Ordered Locus Names:	BUsg_143

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + riboflavin = ADP + FMN. ATP + FMN = diphosphate + FAD.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence similarities	Belongs to the ribF family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Riboflavin biosynthesis protein ribF		PRO_0000194135

Sequences

	Sequence		Length	Mass (Da)
	Q8K9Z1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: AEC2E18F28082DF4	FASTA	312	36,139
	10 20 30 40 50 60 MKLIRGIHNL KKINSNSAVS IGNFDGVHLG HQKLLSNLYK IGKKNNILTV LILFEPQPLE 70 80 90 100 110 120 FLNNKNSPKR LTTIQNKIKY IQSWKIDIIL CIKFNESFSS LSAEKFIKNI LITKLNIKFI 130 140 150 160 170 180 IIGDDFRFGS KRNGNISLLK EIGYQYNFKV IEISSLLYKN KIKISSTNIR KCLLENKIEL 190 200 210 220 230 240 ARKLLGRPFS ISGRVIHGNK IGRKLGYPTA NISLRKNIPL NNGVYAVKIS CFFNKKFVGI 250 260 270 280 290 300 CNIGIKPSYF SSKKHRLLEV HLFNFNLNLY EEKIEVFLYK KIRNECFFSS KNKLKEQISK 310 DIEIVKKYFN LI

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67711.1.
RefSeq	NP_660500.1.
3D structure databases
HSSP	HSSP built from PDB template 1N08 based on UniProtKB O74866.
ModBase	Search...
Genome annotation databases
GeneID	1005960.
GenomeReviews	Gene locus BUsg_143 in contig AE013218_GR.
KEGG	bas:BUsg143.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9Z1.
OMA	Q8K9Z1. GVHLGHR.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG143-MON.
Family and domain databases
InterPro	IPR015864. FAD_synthase. IPR015865. Riboflavin_kinase. IPR002606. Riboflavin_kinase/FAD_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR22749. Riboflavin_kinase. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. PF01687. Flavokinase. 1 hit. [Graphical view]
PIRSF	PIRSF004491. FAD_Synth. 1 hit.
ProDom	PD003662. FAD_Synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00083. ribF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBF_BUCAP

Accession

Primary (citable) accession number: Q8K9Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)