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Q8K9Y9

- DNAJ_BUCAP

UniProt

Q8K9Y9 - DNAJ_BUCAP

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Protein

Chaperone protein DnaJ

Gene
dnaJ, BUsg_145
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity.UniRule annotation

Cofactori

Binds 2 zinc ions per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi146 – 1461Zinc 1 By similarity
Metal bindingi149 – 1491Zinc 1 By similarity
Metal bindingi163 – 1631Zinc 2 By similarity
Metal bindingi166 – 1661Zinc 2 By similarity
Metal bindingi185 – 1851Zinc 2 By similarity
Metal bindingi188 – 1881Zinc 2 By similarity
Metal bindingi199 – 1991Zinc 1 By similarity
Metal bindingi202 – 2021Zinc 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri133 – 21179CR-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein folding Source: InterPro
  3. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-155-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJ
Gene namesi
Name:dnaJ
Ordered Locus Names:BUsg_145
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Chaperone protein DnaJUniRule annotationPRO_0000070746Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg145.

Structurei

3D structure databases

ProteinModelPortaliQ8K9Y9.
SMRiQ8K9Y9. Positions 2-77.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 7066JAdd
BLAST
Repeati146 – 1538CXXCXGXG motifUniRule annotation
Repeati163 – 1708CXXCXGXG motifUniRule annotation
Repeati185 – 1928CXXCXGXG motifUniRule annotation
Repeati199 – 2068CXXCXGXG motifUniRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi77 – 11640Gly-richUniRule annotationAdd
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.
Contains 1 J domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
KOiK03686.
OMAiGHTDPNQ.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9Y9-1 [UniParc]FASTAAdd to Basket

« Hide

MAKKDYYQIL GIPKSAEERE IKKAYKRLAM KYHPDRNQGD KNAENKFKEI    50
KEAYEILINE EKRTAYDQYG HAAFENGYNQ NNTYSTFTSS TDFGDIFGDV 100
FGDIFGGSRN QRVKKGADLC YNMEISLEEA VKGTTKEIRI PTFQKCKTCY 150
GMGTSTGTKP NGCSTCHGKG QIHIRKGFFT VQQSCPTCNG IGTVIKNPCR 200
MCRGQGRIKT NKTLSVKIPP GIDTNDKIRL SKEGEAGTNG AQPGDLYVQM 250
KVNKHPIFER EENNLYCEVP INFTMAALGG EIEVPTLDGR VNLKIPSETQ 300
SGKLFRIRGK GVKSVQSRSR GDLLCRVVVE TPVNLNEKQK YLLSELGNSF 350
NGFRGDKNSP RSKRFFDGVK RFFDDLTK 378
Length:378
Mass (Da):42,469
Last modified:October 1, 2002 - v1
Checksum:iBB73F48DAF7A2D34
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67713.1.
RefSeqiNP_660502.1. NC_004061.1.
WP_011053680.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67713; AAM67713; BUsg_145.
GeneIDi1005962.
KEGGibas:BUsg145.
PATRICi21247071. VBIBucAph100086_0149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67713.1 .
RefSeqi NP_660502.1. NC_004061.1.
WP_011053680.1. NC_004061.1.

3D structure databases

ProteinModelPortali Q8K9Y9.
SMRi Q8K9Y9. Positions 2-77.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198804.BUsg145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM67713 ; AAM67713 ; BUsg_145 .
GeneIDi 1005962.
KEGGi bas:BUsg145.
PATRICi 21247071. VBIBucAph100086_0149.

Phylogenomic databases

eggNOGi COG0484.
KOi K03686.
OMAi GHTDPNQ.
OrthoDBi EOG6BPDKP.

Enzyme and pathway databases

BioCyci BAPH198804:GHMG-155-MONOMER.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPi MF_01152. DnaJ.
InterProi IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiDNAJ_BUCAP
AccessioniPrimary (citable) accession number: Q8K9Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi