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Q8K9Y9

- DNAJ_BUCAP

UniProt

Q8K9Y9 - DNAJ_BUCAP

Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

    Cofactori

    Binds 2 zinc ions per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi146 – 1461Zinc 1UniRule annotation
    Metal bindingi149 – 1491Zinc 1UniRule annotation
    Metal bindingi163 – 1631Zinc 2UniRule annotation
    Metal bindingi166 – 1661Zinc 2UniRule annotation
    Metal bindingi185 – 1851Zinc 2UniRule annotation
    Metal bindingi188 – 1881Zinc 2UniRule annotation
    Metal bindingi199 – 1991Zinc 1UniRule annotation
    Metal bindingi202 – 2021Zinc 1UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri133 – 21179CR-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein folding Source: InterPro
    3. response to heat Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    DNA replication, Stress response

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-155-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chaperone protein DnaJUniRule annotation
    Gene namesi
    Name:dnaJUniRule annotation
    Ordered Locus Names:BUsg_145
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 378378Chaperone protein DnaJPRO_0000070746Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi198804.BUsg145.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K9Y9.
    SMRiQ8K9Y9. Positions 2-77.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 7066JUniRule annotationAdd
    BLAST
    Repeati146 – 1538CXXCXGXG motif
    Repeati163 – 1708CXXCXGXG motif
    Repeati185 – 1928CXXCXGXG motif
    Repeati199 – 2068CXXCXGXG motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi77 – 11640Gly-richAdd
    BLAST

    Domaini

    The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaJ family.UniRule annotation
    Contains 1 CR-type zinc finger.UniRule annotation
    Contains 1 J domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri133 – 21179CR-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0484.
    KOiK03686.
    OMAiGHTDPNQ.
    OrthoDBiEOG6BPDKP.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPiMF_01152. DnaJ.
    InterProiIPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view]
    PfamiPF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K9Y9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKKDYYQIL GIPKSAEERE IKKAYKRLAM KYHPDRNQGD KNAENKFKEI    50
    KEAYEILINE EKRTAYDQYG HAAFENGYNQ NNTYSTFTSS TDFGDIFGDV 100
    FGDIFGGSRN QRVKKGADLC YNMEISLEEA VKGTTKEIRI PTFQKCKTCY 150
    GMGTSTGTKP NGCSTCHGKG QIHIRKGFFT VQQSCPTCNG IGTVIKNPCR 200
    MCRGQGRIKT NKTLSVKIPP GIDTNDKIRL SKEGEAGTNG AQPGDLYVQM 250
    KVNKHPIFER EENNLYCEVP INFTMAALGG EIEVPTLDGR VNLKIPSETQ 300
    SGKLFRIRGK GVKSVQSRSR GDLLCRVVVE TPVNLNEKQK YLLSELGNSF 350
    NGFRGDKNSP RSKRFFDGVK RFFDDLTK 378
    Length:378
    Mass (Da):42,469
    Last modified:October 1, 2002 - v1
    Checksum:iBB73F48DAF7A2D34
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67713.1.
    RefSeqiNP_660502.1. NC_004061.1.
    WP_011053680.1. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM67713; AAM67713; BUsg_145.
    GeneIDi1005962.
    KEGGibas:BUsg145.
    PATRICi21247071. VBIBucAph100086_0149.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67713.1 .
    RefSeqi NP_660502.1. NC_004061.1.
    WP_011053680.1. NC_004061.1.

    3D structure databases

    ProteinModelPortali Q8K9Y9.
    SMRi Q8K9Y9. Positions 2-77.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg145.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM67713 ; AAM67713 ; BUsg_145 .
    GeneIDi 1005962.
    KEGGi bas:BUsg145.
    PATRICi 21247071. VBIBucAph100086_0149.

    Phylogenomic databases

    eggNOGi COG0484.
    KOi K03686.
    OMAi GHTDPNQ.
    OrthoDBi EOG6BPDKP.

    Enzyme and pathway databases

    BioCyci BAPH198804:GHMG-155-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPi MF_01152. DnaJ.
    InterProi IPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    [Graphical view ]
    Pfami PF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiDNAJ_BUCAP
    AccessioniPrimary (citable) accession number: Q8K9Y9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3