Reviewed, UniProtKB/Swiss-Prot Q8K9Y7 (NUOA_BUCAP)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A
    NDH-1 subunit A
    NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	BUsg_147

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	126 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 126	126	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_0000117867
Regions
	Transmembrane	14 – 34	21	Potential
	Transmembrane	66 – 86	21	Potential
	Transmembrane	96 – 116	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K9Y7-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 013593C0DA118096	FASTA	126	14,783
	10 20 30 40 50 60 MLKNTLDMTE YLSFLYFIFF SLFFCCFMLF TSWFLGGRSL SRYKNTPFES GVVSVGNTHL 70 80 90 100 110 120 HFSVKFYLIA IFFVIFDVEA LYLYAWSVSI VESGWIGFVE ATIFILFLLL SLFYLVRIKA LNWVTS

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67715.1.
RefSeq	NP_660504.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005964.
GenomeReviews	Gene locus BUsg_147 in contig AE013218_GR.
KEGG	bas:BUsg147.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9Y7.
OMA	Q8K9Y7. KEIAYES.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG147-MON.
Family and domain databases
HAMAP	MF_01394. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_BUCAP

Accession

Primary (citable) accession number: Q8K9Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)