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Q8K9Y3 (NUOF_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit F

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F
Gene names
Name:nuoF
Ordered Locus Names:BUsg_151
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.

Catalytic activity

NADH + quinone = NAD+ + quinol.

Cofactor

Binds 1 4Fe-4S cluster Probable.

FMN Probable.

Subunit structure

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex By similarity.

Sequence similarities

Belongs to the complex I 51 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 447447NADH-quinone oxidoreductase subunit F
PRO_0000118572

Regions

Nucleotide binding61 – 7010NAD By similarity
Nucleotide binding174 – 22148FMN By similarity

Sites

Metal binding3521Iron-sulfur (4Fe-4S) Potential
Metal binding3551Iron-sulfur (4Fe-4S) Potential
Metal binding3581Iron-sulfur (4Fe-4S) Potential
Metal binding3991Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8K9Y3 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: D11DE0940D67AB51

FASTA44749,865
        10         20         30         40         50         60 
MNNIIPTEET HPLTWRLRND RETVWIEEYC KKNGYFSLKK ALKTMCPEDV VHLIKESGLK 

        70         80         90        100        110        120 
GRGGAGFSTG LKWSLMSKDN SSKIRYLLCN ADEMEPGTYK DRFLMENIPH QLIEGMLLSA 

       130        140        150        160        170        180 
FALNVSRGYI FLRGEYIKAE YILKKSIQEA INFGFIGSNI LNSGFNFELF LHTGAGRYIC 

       190        200        210        220        230        240 
GEETALINSL EGRRANPRAK PPFPAVFGLW GKPTCVNNVE TLSNVPSIVL HGVNWYKKLS 

       250        260        270        280        290        300 
KSTDTTGTKL MGFSGKVNNP GVWELPFGTT AREILEDYAR GMKSGLFLKS WQPGGAGTDF 

       310        320        330        340        350        360 
LIEKHLDLPM DFTSIAKAGS RLGTAIAMAV DNKTNMISLV CNIEKFFSRE SCGLCTPCRE 

       370        380        390        400        410        420 
GLPWIVKILE SLEKKEGHKN DVKNLERLCL DLSPGKTFCA HAPGAVEPLQ SAIKYFRLEF 

       430        440 
EAGISIKKLK KCSNILGIQS NEFTSKV 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67719.1.
RefSeqNP_660508.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9Y3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg151.

Proteomic databases

PRIDEQ8K9Y3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67719; AAM67719; BUsg_151.
GeneID1005968.
KEGGbas:BUsg151.
PATRIC21247083. VBIBucAph100086_0155.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1894.
KOK00335.
OMAHEIGMVP.
OrthoDBEOG6W9XGK.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-161-MONOMER.

Family and domain databases

InterProIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. NADH_UbQ_OxRdtase_51kDa_su.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsTIGR01959. nuoF_fam. 1 hit.
PROSITEPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUOF_BUCAP
AccessionPrimary (citable) accession number: Q8K9Y3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names