Reviewed, UniProtKB/Swiss-Prot Q8K9X3 (FOLC_BUCAP)

Names and origin

Protein names

Recommended name:
    Bifunctional protein folC
Including the following 2 domains:
    1- Recommended name:
            Folylpolyglutamate synthase
              EC=6.3.2.17
        Alternative name(s):
            Folylpoly-gamma-glutamate synthetase
              Short name=FPGS
            Tetrahydrofolylpolyglutamate synthase
              Short name=Tetrahydrofolate synthase
    2- Recommended name:
            Dihydrofolate synthase
              EC=6.3.2.12

Gene names

Name:	folC
Ordered Locus Names:	BUsg_161

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of folates to polyglutamate derivatives.
Catalytic activity	ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1). ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.
Sequence similarities	Belongs to the folylpolyglutamate synthase family.

Ontologies

Keywords
Biological process	Folate biosynthesis One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dihydrofolate synthase activity Inferred from electronic annotation. Source: EC tetrahydrofolylpolyglutamate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Bifunctional protein folC		PRO_0000168301
Regions
	Nucleotide binding	50 – 56	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9X3-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6F644AFF9144AED4	FASTA	418	47,631
	10 20 30 40 50 60 MHKKKYTFSM WMKYLEKFDK KDRKNLFELK LIAKKLGLLN LKSFFFTVGG TNGKGTTCAM 70 80 90 100 110 120 LEKLLLDSGY QVGLYTSPHL INYSERIKVN GLYLSEKDHI FSFLIIDAEK GNVSLTYFEF 130 140 150 160 170 180 ITLSALFLFS QYSLDIIILE VGLGGRLDAT NIIDSDLSVI TNIGIDHTSC LGTDRISIGR 190 200 210 220 230 240 EKSGIFRKGK IAVIGEKNIP ISVDEIAKEK KTILKKIDVD WFWTRTKIDS WNFIHSNIEL 250 260 270 280 290 300 YNLPVSRIPL SNTAIALASL FYSGLEVNLK KLKSSISKVQ LSGRFQTVFN SPRIILDVAH 310 320 330 340 350 360 NVHAALYLSE KIDEIDTEGQ IYAVFGILKD KDVAGVVQVL QKKINYWYVV NLKTNRSASI 370 380 390 400 410 NYLKKKLSLN NALFFNNINE SWQAIKKVIT KKDIILVFGS FFTVSEFMSI KDRRLTLY

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67729.1.
RefSeq	NP_660518.1.
3D structure databases
HSSP	HSSP built from PDB template 1FGS based on UniProtKB P15925.
ModBase	Search...
Genome annotation databases
GeneID	1005359.
GenomeReviews	Gene locus BUsg_161 in contig AE013218_GR.
KEGG	bas:BUsg161.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9X3.
OMA	Q8K9X3. SIHPTEI.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG161-MON.
Family and domain databases
InterPro	IPR018109. Folylpolyglutamate_synth_CS. IPR001645. Fpolygl_synthtse. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
PANTHER	PTHR11136. Fpolygl_synthtse. 1 hit.
Pfam	PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01499. folC. 1 hit.
PROSITE	PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit. PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLC_BUCAP

Accession

Primary (citable) accession number: Q8K9X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)