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Protein

Dihydrofolate synthase/folylpolyglutamate synthase

Gene

folC

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Functions in two distinct reactions of the de novo folate biosynthetic pathway. Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate or 10-formyltetrahydrofolate or 5,10-methylenetetrahydrofolate, leading to folylpolyglutamate derivatives.By similarity

Catalytic activityi

ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate.By similarity
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).By similarity
ATP + 10-formyl-tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + 10-formyl-tetrahydropteroyl-(gamma-Glu)(n+1).By similarity
ATP + 5,10-methylene-tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + 5,10-methylene-tetrahydropteroyl-(gamma-Glu)(n+1).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Dihydrofolate synthase/folylpolyglutamate synthase (folC)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Pathwayi: tetrahydrofolylpolyglutamate biosynthesis

This protein is involved in the pathway tetrahydrofolylpolyglutamate biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolylpolyglutamate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Magnesium 1By similarity1
Metal bindingi140Magnesium 1By similarity1
Metal bindingi167Magnesium 2By similarity1
Binding sitei252ATPBy similarity1
Binding sitei284ATPBy similarity1
Binding sitei297ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 56ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFolate biosynthesis, One-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-173-MONOMER
UniPathwayiUPA00077; UER00157
UPA00850

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate synthase/folylpolyglutamate synthase (EC:6.3.2.12, EC:6.3.2.17)
Short name:
DHFS / FPGS
Alternative name(s):
Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase
Folylpolyglutamate synthetase
Tetrahydrofolylpolyglutamate synthase
Gene namesi
Name:folC
Ordered Locus Names:BUsg_161
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683011 – 418Dihydrofolate synthase/folylpolyglutamate synthaseAdd BLAST418

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi198804.BUsg161

Structurei

3D structure databases

ProteinModelPortaliQ8K9X3
SMRiQ8K9X3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 1197,8-dihydropteroate bindingBy similarity4
Regioni147 – 1497,8-dihydropteroate bindingBy similarity3

Sequence similaritiesi

Belongs to the folylpolyglutamate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105DPM Bacteria
COG0285 LUCA
KOiK11754
OMAiYFEMGTL
OrthoDBiPOG091H021T

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
InterProiView protein in InterPro
IPR001645 Folylpolyglutamate_synth
IPR018109 Folylpolyglutamate_synth_CS
IPR036565 Mur-like_cat_sf
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
PANTHERiPTHR11136 PTHR11136, 1 hit
PfamiView protein in Pfam
PF08245 Mur_ligase_M, 1 hit
PIRSFiPIRSF001563 Folylpolyglu_synth, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
TIGRFAMsiTIGR01499 folC, 1 hit
PROSITEiView protein in PROSITE
PS01011 FOLYLPOLYGLU_SYNT_1, 1 hit
PS01012 FOLYLPOLYGLU_SYNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K9X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKKKYTFSM WMKYLEKFDK KDRKNLFELK LIAKKLGLLN LKSFFFTVGG
60 70 80 90 100
TNGKGTTCAM LEKLLLDSGY QVGLYTSPHL INYSERIKVN GLYLSEKDHI
110 120 130 140 150
FSFLIIDAEK GNVSLTYFEF ITLSALFLFS QYSLDIIILE VGLGGRLDAT
160 170 180 190 200
NIIDSDLSVI TNIGIDHTSC LGTDRISIGR EKSGIFRKGK IAVIGEKNIP
210 220 230 240 250
ISVDEIAKEK KTILKKIDVD WFWTRTKIDS WNFIHSNIEL YNLPVSRIPL
260 270 280 290 300
SNTAIALASL FYSGLEVNLK KLKSSISKVQ LSGRFQTVFN SPRIILDVAH
310 320 330 340 350
NVHAALYLSE KIDEIDTEGQ IYAVFGILKD KDVAGVVQVL QKKINYWYVV
360 370 380 390 400
NLKTNRSASI NYLKKKLSLN NALFFNNINE SWQAIKKVIT KKDIILVFGS
410
FFTVSEFMSI KDRRLTLY
Length:418
Mass (Da):47,631
Last modified:October 1, 2002 - v1
Checksum:i6F644AFF9144AED4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA Translation: AAM67729.1
RefSeqiWP_011053696.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM67729; AAM67729; BUsg_161
KEGGibas:BUsg_161

Entry informationi

Entry nameiFOLC_BUCAP
AccessioniPrimary (citable) accession number: Q8K9X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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