Q8K9X3 (FOLC_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein FolC

Including the following 2 domains:

1. Folylpolyglutamate synthase
   EC=6.3.2.17
   Alternative name(s):
   Folylpoly-gamma-glutamate synthetase
   Short name=FPGS
   Tetrahydrofolylpolyglutamate synthase
   Short name=Tetrahydrofolate synthase
2. Dihydrofolate synthase
   EC=6.3.2.12

Gene names

Name:	folC
Ordered Locus Names:	BUsg_161

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of folates to polyglutamate derivatives.
Catalytic activity	ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1). ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.
Sequence similarities	Belongs to the folylpolyglutamate synthase family.

Ontologies

Keywords
Biological process	Folate biosynthesis One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	dihydrofolate biosynthetic process Inferred from electronic annotation. Source: GOC folic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway tetrahydrofolylpolyglutamate biosynthetic process Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dihydrofolate synthase activity Inferred from electronic annotation. Source: EC tetrahydrofolylpolyglutamate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Bifunctional protein FolC		PRO_0000168301
Regions
	Nucleotide binding	50 – 56	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9X3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6F644AFF9144AED4	FASTA	418	47,631
	10 20 30 40 50 60 MHKKKYTFSM WMKYLEKFDK KDRKNLFELK LIAKKLGLLN LKSFFFTVGG TNGKGTTCAM 70 80 90 100 110 120 LEKLLLDSGY QVGLYTSPHL INYSERIKVN GLYLSEKDHI FSFLIIDAEK GNVSLTYFEF 130 140 150 160 170 180 ITLSALFLFS QYSLDIIILE VGLGGRLDAT NIIDSDLSVI TNIGIDHTSC LGTDRISIGR 190 200 210 220 230 240 EKSGIFRKGK IAVIGEKNIP ISVDEIAKEK KTILKKIDVD WFWTRTKIDS WNFIHSNIEL 250 260 270 280 290 300 YNLPVSRIPL SNTAIALASL FYSGLEVNLK KLKSSISKVQ LSGRFQTVFN SPRIILDVAH 310 320 330 340 350 360 NVHAALYLSE KIDEIDTEGQ IYAVFGILKD KDVAGVVQVL QKKINYWYVV NLKTNRSASI 370 380 390 400 410 NYLKKKLSLN NALFFNNINE SWQAIKKVIT KKDIILVFGS FFTVSEFMSI KDRRLTLY

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67729.1.
RefSeq	NP_660518.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9X3.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg161.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67729; AAM67729; BUsg_161.
GeneID	1005359.
KEGG	bas:BUsg161.
PATRIC	21247103. VBIBucAph100086_0165.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0285.
KO	K11754.
OMA	VICDTGH.
ProtClustDB	CLSK315624.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-231-MONOMER.
UniPathway	UPA00077; UER00157.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR001645. Folylpolyglutamate_synth. IPR018109. Folylpolyglutamate_synth_CS. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. [Graphical view]
PANTHER	PTHR11136. PTHR11136. 1 hit.
Pfam	PF08245. Mur_ligase_M. 1 hit. [Graphical view]
PIRSF	PIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01499. folC. 1 hit.
PROSITE	PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit. PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLC_BUCAP

Accession

Primary (citable) accession number: Q8K9X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program