Reviewed, UniProtKB/Swiss-Prot Q8K9X2 (KPRS_BUCAP)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	BUsg_163

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity HAMAP MF_00583.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141119
Regions
	Region	213 – 226	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	129	1	Magnesium Potential
	Metal binding	131	1	Magnesium Potential
	Metal binding	140	1	Magnesium Potential
	Metal binding	144	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K9X2-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B1B29A51308E5F86	FASTA	315	34,759
	10 20 30 40 50 60 MPDMKLFAGN SIPKLAKFIA HRLYINLGNA AVGRFSDGEI SVQINENVRG SDVFIIQSTC 70 80 90 100 110 120 SPTNDNIMEL VVMVDALRRA SAGRITAVIP YFGYSRQDRR VRSARVPITA KVVADFLSSI 130 140 150 160 170 180 GVDRVLTVDL HAEQIQGFFD VPVDNVFGSL ILLEDMLQRE LKNPIVVSPD IGGVVRARAI 190 200 210 220 230 240 AKLLYDTDMA IIDKRRPRPN ISQIMHIIGD VANRDCILVD DMIDTGGTLC KAAEALKERG 250 260 270 280 290 300 AKRVFAYATH PIFSGKASEN LKKSVIDEVV VCDTIPLEEK IRLLPNVRTL TLAGMLAEAI 310 RRISNEESIS AMFEH

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67730.1.
RefSeq	NP_660519.1.
3D structure databases
SMR	Q8K9X2. Positions 3-312.
ModBase	Search...
Genome annotation databases
GeneID	1005360.
GenomeReviews	Gene locus BUsg_163 in contig AE013218_GR.
KEGG	bas:BUsg163.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG519284.
OMA	YKTAGAD.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG163-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_BUCAP

Accession

Primary (citable) accession number: Q8K9X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)