Q8K9X1 (ISPE_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Synonyms:	ipk
Ordered Locus Names:	BUsg_164

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Subunit structure	Homodimer By similarity. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189198
Regions
	Nucleotide binding	100 – 110	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	142	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9X1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 42A81EAE07F028F3	FASTA	292	32,819
	10 20 30 40 50 60 MINTWPSPAK INLFLYVTGI RSDGYHYIQS LFQFLNYGDT LTIIPNTKGT IELFTESNSL 70 80 90 100 110 120 VNIKNSIITA AELLKEKALH SLGKKTSHFG AKIFLNKKIP IGSGLGGGSS NAATTLVVLN 130 140 150 160 170 180 NLWKTKFTLQ ELAELSLKIG SDIPAFIMGK TTIVEGIGEI LYPIHRKEKW YLIVYPNISI 190 200 210 220 230 240 STKNIFSIYS IKKNTIKKPI DFLLRSRFHN DFENVIKKKF KKIKQLISML SLYAPSRITG 250 260 270 280 290 TGSCIFAEFN DKKSAQKIHS LLPHNVQGTI VKSVNVSPLH HAFYKKNINL FN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67731.1.
RefSeq	NP_660520.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9X1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000235; EBBUCP00000000235; EBBUCG00000000235.
GeneID	1005361.
GenomeReviews	Gene locus BUsg_164 in contig AE013218_GR.
KEGG	bas:BUsg164.
PATRIC	21247109. VBIBucAph100086_0168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008174.
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG164-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BUCAP

Accession

Primary (citable) accession number: Q8K9X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program