Q8K9X0 (RF1_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 1
Short name=RF-1

Gene names

Name:	prfA
Ordered Locus Names:	BUsg_165

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity. HAMAP-Rule MF_00093
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00093.
Post-translational modification	Methylated by PrmC. Methylation increases the termination efficiency of RF1 By similarity. HAMAP-Rule MF_00093
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Methylation
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	translation release factor activity, codon specific Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Peptide chain release factor 1 HAMAP-Rule MF_00093		PRO_0000177647
Amino acid modifications
	Modified residue	235	1	N5-methylglutamine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9X0 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0C69E163BA05D21D	FASTA	361	41,459
	10 20 30 40 50 60 MNSSIFNKLK SLRNRYEEIE IMLSQKDTIS NQEKFKNLSK EYLQISEIVK NFIKWEKLET 70 80 90 100 110 120 DIKNINLLFN DVEMHDIAQE ELFIVKKQKK KLEEKIKILL LPVDPNDQHS CFIEIRAATG 130 140 150 160 170 180 GDESSIFAGE LFRMYTRYAE NYMWKTEIMS SSENERGGFK EIIAKVTGKG ACGRLKFESG 190 200 210 220 230 240 GHRVQRVPET ESQGRIHTST CTVAIMPVRP KTKKEEIKVS DLKIDTFRSS GAGGQHVNTT 250 260 270 280 290 300 DSAIRITHIP SGNVVECQDE RSQHKNKAKA LSILSARVYA DKLAKSQKEN SSMRRILLGT 310 320 330 340 350 360 GERSDRNRTY NFAQNRITDH RINLTIYKLN EVLQGKLDLL IDPIMQEYQA DMLSSLSEFK L

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67732.1.
RefSeq	NP_660521.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9X0.
SMR	Q8K9X0. Positions 8-353.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67732; AAM67732; BUsg_165.
GeneID	1005362.
KEGG	bas:BUsg165.
PATRIC	21247111. VBIBucAph100086_0169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0216.
KO	K02835.
OMA	EMPEINP.
ProtClustDB	PRK00591.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-174-MONOMER.
Family and domain databases
Gene3D	3.30.160.20. 1 hit.
HAMAP	MF_00093. Rel_fac_1.
InterPro	IPR014720. dsRNA-bd-like_dom. IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. IPR004373. PrfA. [Graphical view]
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00019. prfA. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF1_BUCAP

Accession

Primary (citable) accession number: Q8K9X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 29, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program