ID   NADE_BUCAP              Reviewed;         268 AA.
AC   Q8K9W7;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=NH(3)-dependent NAD(+) synthetase;
DE            EC=6.3.1.5;
GN   Name=nadE; OrderedLocusNames=BUsg_168;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP +
CC       diphosphate + NAD(+).
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (ammonia route): step 1/1.
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
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DR   EMBL; AE013218; AAM67735.1; -; Genomic_DNA.
DR   RefSeq; NP_660524.1; -.
DR   HSSP; P08164; 1KQP.
DR   GeneID; 1005365; -.
DR   GenomeReviews; AE013218_GR; BUsg_168.
DR   KEGG; bas:BUsg168; -.
DR   HOGENOM; Q8K9W7; -.
DR   OMA; Q8K9W7; ADLEEDR.
DR   BioCyc; BAPH198804:BUSG168-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0008795; F:NAD+ synthase activity; IEA:EC.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00193; -; 1.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding.
FT   CHAIN         1    268       NH(3)-dependent NAD(+) synthetase.
FT                                /FTId=PRO_0000152161.
FT   NP_BIND      46     53       ATP (By similarity).
FT   ACT_SITE     48     48       By similarity.
SQ   SEQUENCE   268 AA;  30605 MW;  0C81021081F88846 CRC64;
     MTLQKKIIEL LGVKPLIIPK KEIENRIQIL KRYLIENTHL KTLIVGISGG QDSTLTGKLC
     QLSIQELRKE KKEKSYQFIA LRLPYGVQID EKDCRDAINF INPDQIFTIN IKNAVLNSER
     SLKKQGIQIS DYIKGNEKAR ERMKVQYSFA AITNGLVVGT GNAAENVTGF FTKYGDNGTD
     VNLISKLNKR QGKFLLKELN CPKHLYLKKP TADLEDEKPQ KEDEVALGIK YNIIDDYLEG
     KKVNSLNKQI IERLYLTTEH KRKIINLG
//