Reviewed, UniProtKB/Swiss-Prot Q8K9W7 (NADE_BUCAP)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BUsg_168

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152161
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9W7-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0C81021081F88846	FASTA	268	30,605
	10 20 30 40 50 60 MTLQKKIIEL LGVKPLIIPK KEIENRIQIL KRYLIENTHL KTLIVGISGG QDSTLTGKLC 70 80 90 100 110 120 QLSIQELRKE KKEKSYQFIA LRLPYGVQID EKDCRDAINF INPDQIFTIN IKNAVLNSER 130 140 150 160 170 180 SLKKQGIQIS DYIKGNEKAR ERMKVQYSFA AITNGLVVGT GNAAENVTGF FTKYGDNGTD 190 200 210 220 230 240 VNLISKLNKR QGKFLLKELN CPKHLYLKKP TADLEDEKPQ KEDEVALGIK YNIIDDYLEG 250 260 KKVNSLNKQI IERLYLTTEH KRKIINLG

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67735.1.
RefSeq	NP_660524.1.
3D structure databases
HSSP	HSSP built from PDB template 1KQP based on UniProtKB P08164.
ModBase	Search...
Genome annotation databases
GeneID	1005365.
GenomeReviews	Gene locus BUsg_168 in contig AE013218_GR.
KEGG	bas:BUsg168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9W7.
OMA	Q8K9W7. ADLEEDR.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG168-MON.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BUCAP

Accession

Primary (citable) accession number: Q8K9W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)