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Protein

Acetate kinase

Gene

ackA

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg(2+). Can also accept Mn2+.UniRule annotation

Pathway: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131MagnesiumUniRule annotation
Binding sitei20 – 201ATPUniRule annotation
Binding sitei94 – 941SubstrateUniRule annotation
Active sitei153 – 1531Proton donor/acceptorUniRule annotation
Sitei185 – 1851Transition state stabilizerUniRule annotation
Sitei246 – 2461Transition state stabilizerUniRule annotation
Metal bindingi390 – 3901MagnesiumUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi213 – 2175ATPUniRule annotation
Nucleotide bindingi288 – 2903ATPUniRule annotation
Nucleotide bindingi336 – 3405ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-178-MONOMER.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:BUsg_169
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Acetate kinasePRO_0000107539Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg169.

Structurei

3D structure databases

ProteinModelPortaliQ8K9W6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0282.
KOiK00925.
OMAiKIITCHI.
OrthoDBiEOG69975F.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9W6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKILNNLIF VLNCGSSSVK FAILNPDNKK KYLSGLVECL FLSKTYMKWK
60 70 80 90 100
DLTTEHKKCI GSNLSHKDAL NFIFNKFFLE KKDIYKNIVG IGHRVVHGGN
110 120 130 140 150
KIKSSTLIDD NIIQLIQKAI SFAPLHNPAN LIGIKTAIEK FPIFAKKNVA
160 170 180 190 200
VFDTSFYQNM PETSFLYAIP YFFYKEHHIR RYGAHGTSHH YVASEVALIL
210 220 230 240 250
NKNFNSLNVI TCHLGNGASV SAVCNGICVD TSMGLTPLEG LVMGTRSGDI
260 270 280 290 300
DPSIIFFMYQ KLGMSIDEIH TILTKESGLL GLSGISSDFR YFEKKYYFEK
310 320 330 340 350
KARLAVDIFC HRLSKYIASY MSLMENNLDA IVFTGGIGEN VPLIREITLS
360 370 380 390 400
KLLLIGFKID TQRNLGIKNG KHGLITTDKS RPAFVIPTDE ELAIAQETIK

IIK
Length:403
Mass (Da):45,211
Last modified:October 1, 2002 - v1
Checksum:i408D7ABED18A8FA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67736.1.
RefSeqiNP_660525.1. NC_004061.1.
WP_011053703.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67736; AAM67736; BUsg_169.
KEGGibas:BUsg169.
PATRICi21247119. VBIBucAph100086_0173.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67736.1.
RefSeqiNP_660525.1. NC_004061.1.
WP_011053703.1. NC_004061.1.

3D structure databases

ProteinModelPortaliQ8K9W6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg169.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM67736; AAM67736; BUsg_169.
KEGGibas:BUsg169.
PATRICi21247119. VBIBucAph100086_0173.

Phylogenomic databases

eggNOGiCOG0282.
KOiK00925.
OMAiKIITCHI.
OrthoDBiEOG69975F.

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.
BioCyciBAPH198804:GHMG-178-MONOMER.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiACKA_BUCAP
AccessioniPrimary (citable) accession number: Q8K9W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: May 27, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.