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Q8K9W6 (ACKA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetate kinase

EC=2.7.2.1
Alternative name(s):
Acetokinase
Gene names
Name:ackA
Ordered Locus Names:BUsg_169
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction By similarity. HAMAP-Rule MF_00020

Catalytic activity

ATP + acetate = ADP + acetyl phosphate. HAMAP-Rule MF_00020

Cofactor

Mg2+. Can also accept Mn2+ By similarity. HAMAP-Rule MF_00020

Pathway

Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP-Rule MF_00020

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00020

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00020.

Sequence similarities

Belongs to the acetokinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processacetyl-CoA biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

organic acid metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

acetate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403Acetate kinase HAMAP-Rule MF_00020
PRO_0000107539

Regions

Nucleotide binding213 – 2175ATP By similarity
Nucleotide binding288 – 2903ATP By similarity
Nucleotide binding336 – 3405ATP By similarity

Sites

Active site1531Proton donor/acceptor By similarity
Metal binding131Magnesium By similarity
Metal binding3901Magnesium By similarity
Binding site201ATP By similarity
Binding site941Substrate By similarity
Site1851Transition state stabilizer By similarity
Site2461Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9W6 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 408D7ABED18A8FA5

FASTA40345,211
        10         20         30         40         50         60 
MVKILNNLIF VLNCGSSSVK FAILNPDNKK KYLSGLVECL FLSKTYMKWK DLTTEHKKCI 

        70         80         90        100        110        120 
GSNLSHKDAL NFIFNKFFLE KKDIYKNIVG IGHRVVHGGN KIKSSTLIDD NIIQLIQKAI 

       130        140        150        160        170        180 
SFAPLHNPAN LIGIKTAIEK FPIFAKKNVA VFDTSFYQNM PETSFLYAIP YFFYKEHHIR 

       190        200        210        220        230        240 
RYGAHGTSHH YVASEVALIL NKNFNSLNVI TCHLGNGASV SAVCNGICVD TSMGLTPLEG 

       250        260        270        280        290        300 
LVMGTRSGDI DPSIIFFMYQ KLGMSIDEIH TILTKESGLL GLSGISSDFR YFEKKYYFEK 

       310        320        330        340        350        360 
KARLAVDIFC HRLSKYIASY MSLMENNLDA IVFTGGIGEN VPLIREITLS KLLLIGFKID 

       370        380        390        400 
TQRNLGIKNG KHGLITTDKS RPAFVIPTDE ELAIAQETIK IIK 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67736.1.
RefSeqNP_660525.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9W6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg169.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67736; AAM67736; BUsg_169.
GeneID1005366.
KEGGbas:BUsg169.
PATRIC21247119. VBIBucAph100086_0173.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0282.
KOK00925.
OMAKIITCHI.
OrthoDBEOG69975F.
ProtClustDBPRK00180.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-178-MONOMER.
UniPathwayUPA00340; UER00458.

Family and domain databases

HAMAPMF_00020. Acetate_kinase.
InterProIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERPTHR21060. PTHR21060. 1 hit.
PfamPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSPR00471. ACETATEKNASE.
TIGRFAMsTIGR00016. ackA. 1 hit.
PROSITEPS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACKA_BUCAP
AccessionPrimary (citable) accession number: Q8K9W6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names