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Q8K9W3

- RIR1_BUCAP

UniProt

Q8K9W3 - RIR1_BUCAP

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Protein
Ribonucleoside-diphosphate reductase subunit alpha
Gene
nrdA, BUsg_173
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding to separate specificity and activation sites on the alpha subunit. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction. Stimulated by ATP and inhibited by dATP binding to the activity site By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91Allosteric activator By similarity
Binding sitei55 – 551Allosteric activator By similarity
Binding sitei91 – 911Allosteric activator By similarity
Binding sitei209 – 2091Substrate By similarity
Sitei225 – 2251Important for hydrogen atom transfer By similarity
Sitei232 – 2321Allosteric effector binding By similarity
Binding sitei253 – 2531Substrate; via amide nitrogen By similarity
Sitei262 – 2621Allosteric effector binding By similarity
Active sitei437 – 4371Proton acceptor By similarity
Active sitei439 – 4391Cysteine radical intermediate By similarity
Active sitei441 – 4411Proton acceptor By similarity
Sitei462 – 4621Important for hydrogen atom transfer By similarity
Sitei730 – 7301Important for electron transfer By similarity
Sitei731 – 7311Important for electron transfer By similarity
Sitei756 – 7561Interacts with thioredoxin/glutaredoxin By similarity
Sitei761 – 7611Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-182-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:BUsg_173
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 763763Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi225 ↔ 462Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ8K9W3.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi198804.BUsg173.

Structurei

3D structure databases

ProteinModelPortaliQ8K9W3.
SMRiQ8K9W3. Positions 5-737.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 9591ATP-cone
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 217Allosteric activator binding By similarity
Regioni224 – 2252Substrate binding By similarity
Regioni437 – 4415Substrate binding By similarity
Regioni621 – 6255Substrate binding By similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.

Phylogenomic databases

eggNOGiCOG0209.
KOiK00525.
OMAiLLWQMPS.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9W3-1 [UniParc]FASTAAdd to Basket

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MKNSLFVTKR NGKKEKINLD KIHKVLNWAA KGLENISVSQ VELRSRIQFY    50
NNISTVNIHE TIIKAAADLI SENTPDYQYM AARLAIFHLR KKAYGQFEPP 100
NLYYHVKKMV QLGKYDEKLL QNYSFEEYVE MNSFVDHRRD MNFSYAAVKQ 150
LEAKYLLQNR VTGQIYESAQ FLYILISACL FSKYEKKVRM NYIQRFYNAI 200
STFKISLPTP IMSGVRTPTR QFSSCVLIEC ADNLNSINAT TSAIVKYVSQ 250
RAGIGINAGQ IRALGSPIRN GEAFHTGCIP FYKHFQSAVK SCSQGGVRGG 300
AATVFYPIWH LEIESLLVLK NNRGIEENRV RHLDYAVQIN KLMYQRMLLG 350
QKITLFSPSD VPKLYEFFFS DQKKFKEIYI KYEKNRNIRK KSINAIDLFC 400
LIMRERASTG RIYIQHVDHC NSHSAFNSKL ATIRQSNLCL EITLPTKPLN 450
NIDDKNGEIA LCTLSALNLG LINDLHDLKE LSTLSVRALD EILEYQNYPV 500
VCAKKSAISR RSLGIGVINF AYYLAKNKVR YSDGSAKNLT HKTFEAIQYY 550
LLKASCELAK EKGSCSLFNQ TNYYLGKFPI DTYKKDIDSI CNEPLHLNWN 600
LLRKKIKKYG LRNSTLSALM PSETSSQISN ATNGIEPPRG FISIKSSKDG 650
MLRQVVPEYK KLKSEYELLW EIPNNIGYLE LVAIMQKFVD QSISANTNYD 700
PKRFLNEKIP MKQLIYDLLV AYKLGIKTLY YQNTRDGAED NQNLNKSIEN 750
IKEDNCTSGS CTI 763
Length:763
Mass (Da):87,471
Last modified:October 1, 2002 - v1
Checksum:iBDE02289A8CF8D3D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67739.1.
RefSeqiNP_660528.1. NC_004061.1.
WP_011053706.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67739; AAM67739; BUsg_173.
GeneIDi1005370.
KEGGibas:BUsg173.
PATRICi21247127. VBIBucAph100086_0177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67739.1 .
RefSeqi NP_660528.1. NC_004061.1.
WP_011053706.1. NC_004061.1.

3D structure databases

ProteinModelPortali Q8K9W3.
SMRi Q8K9W3. Positions 5-737.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198804.BUsg173.

Proteomic databases

PRIDEi Q8K9W3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM67739 ; AAM67739 ; BUsg_173 .
GeneIDi 1005370.
KEGGi bas:BUsg173.
PATRICi 21247127. VBIBucAph100086_0177.

Phylogenomic databases

eggNOGi COG0209.
KOi K00525.
OMAi LLWQMPS.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci BAPH198804:GHMG-182-MONOMER.

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiRIR1_BUCAP
AccessioniPrimary (citable) accession number: Q8K9W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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