Reviewed, UniProtKB/Swiss-Prot Q8K9W3 (RIR1_BUCAP)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase

Gene names

Name:	nrdA
Ordered Locus Names:	BUsg_173

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family. Contains 1 ATP-cone domain.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	Ribonucleoside-diphosphate reductase subunit alpha		PRO_0000187207
Regions
	Domain	5 – 95	91	ATP-cone
Sites
	Active site	225	1	Hydrogen atom transfer By similarity
	Active site	437	1	Proton acceptor By similarity
	Active site	439	1	Proton acceptor By similarity
	Active site	441	1	Proton acceptor By similarity
	Active site	462	1	Hydrogen atom transfer By similarity
	Active site	730	1	Electron transfer By similarity
	Active site	731	1	Electron transfer By similarity
	Site	232	1	Allosteric effector binding By similarity
	Site	262	1	Allosteric effector binding By similarity
	Site	756	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	761	1	Interacts with thioredoxin/glutaredoxin By similarity
Amino acid modifications
	Disulfide bond	225 ↔ 462		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9W3-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: BDE02289A8CF8D3D	FASTA	763	87,471
	10 20 30 40 50 60 MKNSLFVTKR NGKKEKINLD KIHKVLNWAA KGLENISVSQ VELRSRIQFY NNISTVNIHE 70 80 90 100 110 120 TIIKAAADLI SENTPDYQYM AARLAIFHLR KKAYGQFEPP NLYYHVKKMV QLGKYDEKLL 130 140 150 160 170 180 QNYSFEEYVE MNSFVDHRRD MNFSYAAVKQ LEAKYLLQNR VTGQIYESAQ FLYILISACL 190 200 210 220 230 240 FSKYEKKVRM NYIQRFYNAI STFKISLPTP IMSGVRTPTR QFSSCVLIEC ADNLNSINAT 250 260 270 280 290 300 TSAIVKYVSQ RAGIGINAGQ IRALGSPIRN GEAFHTGCIP FYKHFQSAVK SCSQGGVRGG 310 320 330 340 350 360 AATVFYPIWH LEIESLLVLK NNRGIEENRV RHLDYAVQIN KLMYQRMLLG QKITLFSPSD 370 380 390 400 410 420 VPKLYEFFFS DQKKFKEIYI KYEKNRNIRK KSINAIDLFC LIMRERASTG RIYIQHVDHC 430 440 450 460 470 480 NSHSAFNSKL ATIRQSNLCL EITLPTKPLN NIDDKNGEIA LCTLSALNLG LINDLHDLKE 490 500 510 520 530 540 LSTLSVRALD EILEYQNYPV VCAKKSAISR RSLGIGVINF AYYLAKNKVR YSDGSAKNLT 550 560 570 580 590 600 HKTFEAIQYY LLKASCELAK EKGSCSLFNQ TNYYLGKFPI DTYKKDIDSI CNEPLHLNWN 610 620 630 640 650 660 LLRKKIKKYG LRNSTLSALM PSETSSQISN ATNGIEPPRG FISIKSSKDG MLRQVVPEYK 670 680 690 700 710 720 KLKSEYELLW EIPNNIGYLE LVAIMQKFVD QSISANTNYD PKRFLNEKIP MKQLIYDLLV 730 740 750 760 AYKLGIKTLY YQNTRDGAED NQNLNKSIEN IKEDNCTSGS CTI

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67739.1.
RefSeq	NP_660528.1.
3D structure databases
HSSP	HSSP built from PDB template 1R1R based on UniProtKB P00452.
SMR	Q8K9W3. Positions 4-737.
ModBase	Search...
Genome annotation databases
GeneID	1005370.
GenomeReviews	Gene locus BUsg_173 in contig AE013218_GR.
KEGG	bas:BUsg173.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9W3.
OMA	ATLFYPL.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG173-MON.
Family and domain databases
InterPro	IPR005144. ATP-cone. IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF03477. ATP-cone. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS51161. ATP_CONE. 1 hit. PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_BUCAP

Accession

Primary (citable) accession number: Q8K9W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)