Q8K9W2 (GYRA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	BUsg_174

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 825	825	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145224
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9W2 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 46130AAE8311219E	FASTA	825	93,365
	10 20 30 40 50 60 MKHLAREIVQ VDIEKELKSS YLDYAMSVIV GRALPDVRDG LKPVHRRILF AMHVLSNYWN 70 80 90 100 110 120 KAYKKSARVV GDVIGKYHPH GDSAVYDSIV RMAQNFSLRY MLIDGQGNFG SIDGDAAAAM 130 140 150 160 170 180 RYTEVRMSKI SQELLADLEK NTVEFIPNYD GTEHIPEILP SKIPNLLING SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLNEV IDGCLAYIKD DNITLRELIQ HIPGPDFPTA GIINGKKGIE EAYRTGKGKI 250 260 270 280 290 300 YIRARNKIEK NKKNKKESII FYEIPYQVNK SRLIEKIAEL VKEKRIDGIT ALRDESDKDG 310 320 330 340 350 360 MRIVVEIKKE AMSEIILNQL YSLTQLQISF GINMVALCQG QPKTLSLKEI LKHFLSHREE 370 380 390 400 410 420 IVTRRSIFEL DKTCKRSHIL EGLSIALINI NCIIDLIKKA KSSLEAKELI IKKTWIYKNS 430 440 450 460 470 480 EKINQKDHDY HFTEEQVQAI LDLRLHKLTS LEKNKLVLEY DCLTKKIKEL KKILKSRDRM 490 500 510 520 530 540 REVIRDELVS IKNNFGDQRR TEITENNPDI SIEDLINQED VVVTLSHSGY VKYQPLSDYN 550 560 570 580 590 600 AQKRGGKGKS AAKIKEEDFI ESLVVTNTHD TILCFSSRGI LYWMKVYQLP ESSRHAKGRP 610 620 630 640 650 660 IVNLLPLSNK ERITAILPIH EFKDNLNIFM ATAQGIVKKS SLKEFKKPRI AGIIAINLRA 670 680 690 700 710 720 NDELIGVSLT NGKNNIMLFT QNGKVVQFLE NSVRTMGRTA SGVRGIKISK NDKVVSLIVP 730 740 750 760 770 780 YKKESILIAT NNGYGKRTKI TDFPIKSRAT QGVISIKITK KNGKIIGAIQ VVEKDQIMMI 790 800 810 820 TNAGTLVRIR ASEIGVLKRN TQGVILIRTS KNEKVVALQR IVEPI

References

[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67740.1.
RefSeq	NP_660529.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9W2.
SMR	Q8K9W2. Positions 30-504, 518-823.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000275; EBBUCP00000000275; EBBUCG00000000275.
GeneID	1005371.
GenomeReviews	Gene locus BUsg_174 in contig AE013218_GR.
KEGG	bas:BUsg174.
PATRIC	21247129. VBIBucAph100086_0178.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007782.
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG174-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 2 hits.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_BUCAP

Accession

Primary (citable) accession number: Q8K9W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program