Reviewed, UniProtKB/Swiss-Prot Q8K9V0 (KHSE_BUCAP)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BUsg_187

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Homoserine kinase HAMAP MF_00384		PRO_0000156556
Regions
	Nucleotide binding	91 – 101	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K9V0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 76118A4C8CD1C3D3	FASTA	309	34,036
	10 20 30 40 50 60 MIKIYAPASV ANVGVGFDIL GAAIIPINGT LLGDCVTVKL SKKFELINKG IFSNKLPINK 70 80 90 100 110 120 EQNIVWKCWF KFCKVIKKNI PVSIILEKNM PIGSGLGSSA CSVVATLVAI NQICNNPLNS 130 140 150 160 170 180 KELLLLMGEI EGEISGSIHY DNVAPSYLGG LQLILEDSDI ISQKIPNFKH WFWIIAWPGI 190 200 210 220 230 240 KVSTAEARKI LPKEYKKDIC IKNSRYLAGF IHASYTKQSH LAARCMQDFI AEPYRSKLLP 250 260 270 280 290 300 NFLNAKEKIK KIGAISCGIS GSGPTIFAIS DKIKTAEKIS LWLKNNYLQN KTGFVHICAV DVKGVRKIG

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67752.1.
RefSeq	NP_660541.1.
3D structure databases
HSSP	HSSP built from PDB template 1H74 based on UniProtKB Q58504.
ModBase	Search...
Genome annotation databases
GeneID	1005384.
GenomeReviews	Gene locus BUsg_187 in contig AE013218_GR.
KEGG	bas:BUsg187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9V0.
OMA	Q8K9V0. CGSAHAD.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG187-MON.
Family and domain databases
HAMAP	MF_00384. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR014721. Ribosomal_S5_D2-type_fold. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BUCAP

Accession

Primary (citable) accession number: Q8K9V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)