Q8K9U3 (SECA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein translocase subunit SecA

Gene names

Name:	secA
Ordered Locus Names:	BUsg_195

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane By similarity. HAMAP-Rule MF_01382
Cofactor	May bind 1 zinc ion per subunit Potential.
Subunit structure	Monomer and homodimer By similarity. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm By similarity. Note: Distribution is 50-50 By similarity. HAMAP-Rule MF_01382
Sequence similarities	Belongs to the SecA family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular protein transmembrane transport Inferred from electronic annotation. Source: HAMAP protein import Inferred from electronic annotation. Source: InterPro protein targeting Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 874	874	Protein translocase subunit SecA HAMAP-Rule MF_01382		PRO_0000109579
Regions
	Nucleotide binding	102 – 109	8	ATP Potential
Sites
	Metal binding	859	1	Zinc Potential
	Metal binding	861	1	Zinc Potential
	Metal binding	870	1	Zinc Potential
	Metal binding	871	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K9U3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: BC49815F42051791	FASTA	874	101,164
	10 20 30 40 50 60 MFIKFLHKIF SNRNDRILKK FKKIVSSINQ LEEKFKKLSD KKLQENTGIF RLRLKKGECL 70 80 90 100 110 120 DDLLPESFAT VREASRRVFN MRHFDVQILG GIVLNKQCIA EMRTGEGKTL TSTLPAYLNA 130 140 150 160 170 180 LTGRGVHIVT MNDYLAERDA KNNTPLFEFL GLTVGLNLPE MSFIDKKKAY LCDITYGTNN 190 200 210 220 230 240 EYGFDYLRDN MIFSAEERVQ RELNYALIDE VDSILIDEAR TPLIISGPSE DSSFLYKEIN 250 260 270 280 290 300 KLVPSLICQK KEDSDKFHGN GHFSIDEKSK QIYLTERGLV EVEKILLDRK LMKKEESLYS 310 320 330 340 350 360 SNNIILMHHV ISALRAHNLF TRNIDYLVKD NNIIIVDEHT GRTMPGRRWS DGLHQAIEAK 370 380 390 400 410 420 ENVTVRNENQ TLASITFQNY FRLYKKIAGM TGTAATESFE FSSIYNLDTV IIPPNKPMIR 430 440 450 460 470 480 KDLSDLVYMT EEEKINAILK DIKNCIKKNQ PVLVGTISIE KSEMISKKLK ILNIKHNVLN 490 500 510 520 530 540 AKFHAREAEI IAQAGKPKSV TIATNMAGRG TDIVLGGSLE SQLEKNMYLD KIETIKRNWK 550 560 570 580 590 600 KQHDLVVLSG GLHIIGTERH ESRRIDNQLR GRSGRQGDSG SSRFYLSMED SLMRIFASDK 610 620 630 640 650 660 IISMMRKLGL SLNEAIEHPW VTKAIENAQK KVENRNFDIR KQLLEYDDVC NEQRRVIYAQ 670 680 690 700 710 720 RNKLIDSENI QQNIYDILKD VLHSIIKTHL NFDFPKNTRN ILDLENKLSI EFNLNISIKD 730 740 750 760 770 780 WLKKDHDIKK ENIIKKIIDI AKKNYLNKEI QIGFHNIRMI EKSIMLKTLD SLWKEHLSAM 790 800 810 820 830 840 DYLRQGIHLR GYAQKDPKQE YKRESFNMFS NMLELLKYEV ISFLSKLDIS YIKSNLHLNM 850 860 870 NNNSSIVNND IKMGRNTPCF CKSGKKYKYC HGSL

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67759.1.
RefSeq	NP_660548.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9U3.
SMR	Q8K9U3. Positions 9-829.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg195.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67759; AAM67759; BUsg_195.
GeneID	1005392.
KEGG	bas:BUsg195.
PATRIC	21247177. VBIBucAph100086_0202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0653.
KO	K03070.
OMA	ERFFILQ.
ProtClustDB	PRK12904.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-261-MONOMER.
Family and domain databases
Gene3D	1.10.3060.10. 1 hit. 3.90.1440.10. 1 hit.
HAMAP	MF_01382. SecA.
InterPro	IPR004027. SEC_C_motif. IPR000185. SecA. IPR020937. SecA_CS. IPR011115. SecA_DEAD. IPR014018. SecA_motor_DEAD. IPR011130. SecA_preprotein_X-link_dom. IPR011116. SecA_Wing/Scaffold. [Graphical view]
Pfam	PF02810. SEC-C. 1 hit. PF07517. SecA_DEAD. 1 hit. PF01043. SecA_PP_bind. 1 hit. PF07516. SecA_SW. 1 hit. [Graphical view]
PRINTS	PR00906. SECA.
SMART	SM00957. SecA_DEAD. 1 hit. SM00958. SecA_PP_bind. 1 hit. [Graphical view]
SUPFAM	SSF81767. SecA_PP_bd. 1 hit. SSF81886. SecA_SW. 1 hit.
TIGRFAMs	TIGR00963. secA. 1 hit.
PROSITE	PS01312. SECA. 1 hit. PS51196. SECA_MOTOR_DEAD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SECA_BUCAP

Accession

Primary (citable) accession number: Q8K9U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program