Reviewed, UniProtKB/Swiss-Prot Q8K9U0 (GUAC_BUCAP)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	BUsg_198

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_00596
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	GMP reductase HAMAP MF_00596		PRO_0000093733
Regions
	Nucleotide binding	108 – 131	24	NADP By similarity
	Nucleotide binding	216 – 239	24	NADP By similarity
Sites
	Active site	186	1	Thioimidate intermediate By similarity
	Metal binding	181	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	183	1	Potassium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9U0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D5CCF618376665CF	FASTA	349	38,618
	10 20 30 40 50 60 MRIEEDIKLG FKDVLIRPKR STLKSRSEVN LIRCFSFKYS TMKWFGIPLI AANMDTIGTF 70 80 90 100 110 120 RMAEALSKFN ILTAVHKYYS FDEWKNFISV SSKEILEHVI VSIGTSNLDF FKIKKIFSLS 130 140 150 160 170 180 SELKYICIDV ANGYSEHFVS FLKKIRSFFP DKIICAGNVV TGEMVEELIL SGADIVKVGI 190 200 210 220 230 240 GPGSVCTTRL KTGIGYPQLS AIIECADAAH GLNGQIISDG GCTVSGDIAK AFGGGADFVM 250 260 270 280 290 300 LGGMFAGHTE CLGEIIQEKS KKFMLFYGMS STSAMKRYTG KIPGYRASEG KIVKIPFRGN 310 320 330 340 VDVTVRDILG GLRSSCTYVG AQKLKELTKR TTFIRVSEQE NCIFNNFKN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67762.1.
RefSeq	NP_660551.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3O based on UniProtKB P12268.
ModBase	Search...
Genome annotation databases
GeneID	1005395.
GenomeReviews	Gene locus BUsg_198 in contig AE013218_GR.
KEGG	bas:BUsg198.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9U0.
OMA	Q8K9U0. HTLFTAV.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG198-MON.
Family and domain databases
HAMAP	MF_00596. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_BUCAP

Accession

Primary (citable) accession number: Q8K9U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)