Reviewed, UniProtKB/Swiss-Prot Q8K9T1 (AMPM_BUCAP)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	BUsg_224

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Methionine aminopeptidase		PRO_0000148930
Sites
	Metal binding	96	1	Cobalt 1 By similarity
	Metal binding	107	1	Cobalt 1 By similarity
	Metal binding	107	1	Cobalt 2 By similarity
	Metal binding	170	1	Cobalt 2 By similarity
	Metal binding	202	1	Cobalt 2 By similarity
	Metal binding	233	1	Cobalt 1 By similarity
	Metal binding	233	1	Cobalt 2 By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9T1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A2B7009D05AF2EAE	FASTA	261	29,553
	10 20 30 40 50 60 MNCIIKTESE IKKMKVSGKI AAEVLEMIEN YIKPNISTEE INNICHNFII KKKAVSACLG 70 80 90 100 110 120 YHGFPKSICI SVNDVVCHGI PNKNQILKSG DIVNIDVTII KKNYHADTSK MFLVGQTNIL 130 140 150 160 170 180 SQRLCKIAQE SLYKSLNILK PGIPLYKIGE VIQNYVENNN FSVVKEYCGH GIGRAFHEEP 190 200 210 220 230 240 YVLHYKNKSH VILEKGMIFT IEPMINAGSH EVKCMKDGWT VKTKDHSLSA QYEHTVLITE 250 260 NGCDILTWQK NEKIPSKFIN K

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67783.1.
RefSeq	NP_660572.1.
3D structure databases
HSSP	HSSP built from PDB template 1C24 based on UniProtKB P07906.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Genome annotation databases
GeneID	1005423.
GenomeReviews	Gene locus BUsg_224 in contig AE013218_GR.
KEGG	bas:BUsg224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9T1.
OMA	VIQKHAE.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG224-MON.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_BUCAP

Accession

Primary (citable) accession number: Q8K9T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)